Human uPAR ELISA Kit
Key features and details
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- 反应物种:
- 应用:
- 特异性:
- 靶点名称:
- 偶联标记:
- 偶联标记说明:
- 灵敏度:
- 样品类型:
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Brand:
CAT.NO. : ARG81423
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Product Details
内含物
| 货号 | 内含物名称 | 包装 | 温度 |
|---|---|---|---|
| ARG81423-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81423-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG81423-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81423-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81423-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81423-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81423-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81423-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81423-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81423-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81423-011 | Plate sealer | 4 strips | Room temperature |
概述
| 产品描述 | ARG81423 Human uPAR ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human uPAR in serum, plasma (heparin, EDTA), urine and cell culture supernatants. |
|---|---|
| 反应物种 | Hu |
| 应用 | ELISA |
| 特异性 | There is no detectable cross-reactivity with other relevant proteins. |
| 靶点名称 | uPAR |
| 偶联标记 | HRP |
| 偶联标记说明 | Substrate: TMB and read at 450 nm. |
| 灵敏度 | 31.25 pg/ml |
| 样品类型 | Serum, plasma (heparin, EDTA), urine and cell culture supernatants. |
| 标准范围 | 62.5 - 4000 pg/ml |
| 样本量 | 100 µl |
| 精确 | Intra-Assay CV: 6.4% Inter-Assay CV: 7.5% |
| 別名 | Monocyte activation antigen Mo3; CD antigen CD87; uPAR; U-PAR; URKR; Urokinase plasminogen activator surface receptor; UPAR; CD87 |
应用说明
| 检测时间 | ~ 5 hours |
|---|
属性
| 形式 | 96 well |
|---|---|
| 存放说明 | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | Swiss-port # Q03405 Human Urokinase plasminogen activator surface receptor |
|---|---|
| 基因名称 | PLAUR |
| 全名 | plasminogen activator, urokinase receptor |
| 背景介绍 | This gene encodes the receptor for urokinase plasminogen activator and, given its role in localizing and promoting plasmin formation, likely influences many normal and pathological processes related to cell-surface plasminogen activation and localized degradation of the extracellular matrix. It binds both the proprotein and mature forms of urokinase plasminogen activator and permits the activation of the receptor-bound pro-enzyme by plasmin. The protein lacks transmembrane or cytoplasmic domains and may be anchored to the plasma membrane by a glycosyl-phosphatidylinositol (GPI) moiety following cleavage of the nascent polypeptide near its carboxy-terminus. However, a soluble protein is also produced in some cell types. Alternative splicing results in multiple transcript variants encoding different isoforms. The proprotein experiences several post-translational cleavage reactions that have not yet been fully defined. [provided by RefSeq, Jul 2008] |
| 生物功能 | Acts as a receptor for urokinase plasminogen activator. Plays a role in localizing and promoting plasmin formation. Mediates the proteolysis-independent signal transduction activation effects of U-PA. It is subject to negative-feedback regulation by U-PA which cleaves it into an inactive form. [UniProt] |
| 产品亮点 | Related products: uPAR antibodies; uPAR ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
检测图片 (1)
ARG81423 Human uPAR ELISA Kit standard curve image
ARG81423 Human uPAR ELISA Kit results of a typical standard run with optical density reading at 450 nm.
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