Human VE Cadherin ELISA Kit

Protocol Booklet

Key features and details

Specification: 96 Test
Sensitivity: 0.01 ng/ml (50 μl)；0.12 ng/ml (10 μl)
Standard Curve Range: 0.14~100 ng/ml
Standard Curve Gradient: 7 Points/3 Folds
Number of Incubations: 2
Sample Volume: 50 μl/10 μl
Assay type: Sandwich Elisa
Operation Duration: 120min

Brand:

CAT.NO. : AEH0091

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Size:

96T

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Product Details

Background

Vascular endothelial (VE) - cadherin (VE-CAD), also called 7B4 and cadherin-5, is a member of the cadherin family of cell adhesion molecules. Cadherins are calcium‑dependent transmembrane proteins which bind to one another in a homophilic manner. On their cytoplasmic side, they associate with the three catenins, alpha, beta, and gamma (plakoglobin). This association links the cadherin protein to the cytoskeleton. Without association with the catenins, the cadherins are non-adhesive. Cadherins play a role in development, specifically in tissue formation. They may also help to maintain tissue architecture in the adult. VE-Cadherin has been shown to play important roles in vasculogenesis and angiogenesis. VE-cadherin is a classical cadherin molecule. Classical cadherins consist of a large extracellular domain which contains DXD and DXNDN repeats responsible for mediating calcium‑dependent adhesion, a single-pass transmembrane domain, and a short carboxy-terminal cytoplasmic domain responsible for interacting with the catenins. Human VE-cadherin is a 784 amino acid (aa) residue protein with a 25 aa signal sequence and a 759 aa propeptide. The mature protein begins at amino acid 48 and has a 546 aa extracellular domain, a 27 aa transmembrane domain, and a 164 aa cytoplasmic domain. The human and mouse mature VE-CAD proteins share approximately 74% homology.

Typical Data

pg/ml	O.D.		Average	Corrected
0.00	0.0176	0.0176	0.0176
0.14	0.0228	0.0224	0.0226	0.0050
0.41	0.0323	0.0328	0.0326	0.0150
1.23	0.0583	0.0614	0.0599	0.0423
3.70	0.1408	0.1456	0.1432	0.1256
11.11	0.3962	0.3948	0.3955	0.3779
33.33	1.0930	1.1360	1.1145	1.0969
100.00	2.8490	2.8830	2.8660	2.8484

Precision

	Intra-assay Precision			Inter-assay Precision
Sample Number	S1	S2	S3	S1	S2	S3
Sample Number	22	22	22	6	6	6
Average（ng/ml）	37.7	10.4	2.0	32.5	9.0	1.8
Standard deviation	1.8	0.5	0.1	0.9	0.3	0.1
Coefficient of variation（%）	4.9	4.7	3.5	2.7	3.3	3.3

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)Three samples of known concentration were tested six times on one plate to assess intra-assay precision.

Spike Recovery

The spike recovery was evaluated by spiking 3 levels of human VE Cadherin into health rat serum sample. The un-spiked serum was used as blank in this experiment.

The recovery ranged from 79% to 122% with an overall mean recovery of 98% .

Sample Values

Sample Matrix

Sample Evaluated

Range (ng/ml)

Detectable

Mean of Detectable (ng/ml)

Serum

n.d.-0.18

18.8

0.13

Serum/Plasma - Thirty samples from apparently healthy human were evaluated for the presence of human VE Cadherin in this assay. No medical histories were available for the donors.

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