Human VEGFC ELISA Kit
Key features and details
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- 反应物种:
- 应用:
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- 靶点名称:
- 偶联标记:
- 偶联标记说明:
- 灵敏度:
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Brand:
CAT.NO. : ARG81430
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Product Details
内含物
| 货号 | 内含物名称 | 包装 | 温度 |
|---|---|---|---|
| ARG81430-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81430-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG81430-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81430-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81430-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81430-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81430-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81430-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81430-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81430-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81430-011 | Plate sealer | 4 strips | Room temperature |
概述
| 产品描述 | ARG81430 Human VEGFC ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human VEGFC in serum, plasma (heparin, EDTA) and cell culture supernatants. |
|---|---|
| 反应物种 | Hu |
| 应用 | ELISA |
| 特异性 | There is no detectable cross-reactivity with other relevant proteins. |
| 靶点名称 | VEGFC |
| 偶联标记 | HRP |
| 偶联标记说明 | Substrate: TMB and read at 450 nm. |
| 灵敏度 | 31.25 pg/ml |
| 样品类型 | Serum, plasma (heparin, EDTA) and cell culture supernatants. |
| 标准范围 | 62.5 - 4000 pg/ml |
| 样本量 | 100 µl |
| 精确 | Intra-Assay CV: 5.6% Inter-Assay CV: 6.8% |
| 別名 | VRP; Flt4-L; VEGF-C; Vascular endothelial growth factor-related protein; Flt4 ligand; Vascular endothelial growth factor C; LMPH1D |
应用说明
| 检测时间 | ~ 5 hours |
|---|
属性
| 形式 | 96 well |
|---|---|
| 存放说明 | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | Swiss-port # P49767 Human Vascular endothelial growth factor C |
|---|---|
| 基因名称 | VEGFC |
| 全名 | vascular endothelial growth factor C |
| 背景介绍 | The protein encoded by this gene is a member of the platelet-derived growth factor/vascular endothelial growth factor (PDGF/VEGF) family. The encoded protein promotes angiogenesis and endothelial cell growth, and can also affect the permeability of blood vessels. The proprotein is further cleaved into a fully processed form that can bind and activate VEGFR-2 and VEGFR-3 receptors. [provided by RefSeq, Apr 2014] |
| 生物功能 | Growth factor active in angiogenesis, and endothelial cell growth, stimulating their proliferation and migration and also has effects on the permeability of blood vessels. May function in angiogenesis of the venous and lymphatic vascular systems during embryogenesis, and also in the maintenance of differentiated lymphatic endothelium in adults. Binds and activates VEGFR-2 (KDR/FLK1) and VEGFR-3 (FLT4) receptors. [UniProt] |
| 产品亮点 | Related products: VEGF antibodies; VEGF ELISA Kits; VEGF Duos / Panels; VEGF recombinant proteins; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| 翻译后修饰 | Undergoes a complex proteolytic maturation which generates a variety of processed secreted forms with increased activity toward VEGFR-3, but only the fully processed form could activate VEGFR-2. VEGF-C first form an antiparallel homodimer linked by disulfide bonds. Before secretion, a cleavage occurs between Arg-227 and Ser-228 producing a heterotetramer. The next extracellular step of the processing removes the N-terminal propeptide. Finally the mature VEGF-C is composed mostly of two VEGF homology domains (VHDs) bound by non-covalent interactions. [UniProt] |
检测图片 (1)
ARG81430 Human VEGFC ELISA Kit standard curve image
ARG81430 Human VEGFC ELISA Kit results of a typical standard run with optical density reading at 450 nm.
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