Human VEGFR2/KDR ELISA Kit
Key features and details
- Specification:
- Sensitivity:
- Standard Curve Range:
- Standard Curve Gradient:
- Number of Incubations:
- Sample Volume:
- Assay type:
- Operation Duration:
-
Brand:
CAT.NO. : AEH0200
RMB Please choose
RMB Please choose
Size:
Trail, Bulk size or Custom requests Please contact us
Product Details
Background
VEGF R1 (Flt-1), VEGF R2 (KDR/Flk-1), and VEGF R3 (Flt-4) belong to the class III subfamily of receptor tyrosine kinases (RTKs). All three receptors contain seven immunoglobulin-like repeats in their extracellular domain and kinase insert domains in their intracellular region. They are best known for regulating VEGF family-mediated vasculogenesis, angiogenesis, and lymphangiogenesis. They are also mediators of neurotrophic activity and regulators of hematopoietic development. VEGF R2 is thought to be the primary inducer of VEGF-mediated blood vessel growth, while VEGF R3 plays a significant role in VEGF-C and VEGF-D-mediated lymphangiogenesis.
Typical data
|
pg/ml |
O.D. |
Average |
Corrected |
|
|
0.00 |
0.0221 |
0.0195 |
0.0208 |
|
|
6.86 |
0.0336 |
0.0305 |
0.0321 |
0.0113 |
|
20.58 |
0.0500 |
0.0446 |
0.0473 |
0.0265 |
|
61.73 |
0.1072 |
0.1073 |
0.1073 |
0.0865 |
|
185.19 |
0.2788 |
0.2483 |
0.2636 |
0.2428 |
|
555.56 |
0.7927 |
0.7507 |
0.7717 |
0.7509 |
|
1666.67 |
2.1021 |
2.0794 |
2.0908 |
2.0700 |
|
5000.00 |
4.1378 |
4.1878 |
4.1628 |
4.1420 |
Precision
|
Intra-assay Precision |
Inter-assay Precision |
|||||
|
Sample Number |
S1 |
S2 |
S3 |
S1 |
S2 |
S3 |
|
22 |
22 |
22 |
6 |
6 |
6 |
|
|
Average(pg/ml) |
121.47 |
525.25 |
1597.38 |
116.40 |
515.89 |
1636.98 |
|
Standard Deviation |
6.01 |
32.20 |
84.55 |
1.94 |
15.10 |
85.29 |
|
Coefficient of Variation(%) |
4.9 |
6.1 |
5.3 |
1.7 |
2.9 |
5.2 |
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human VEGFR2/KDR into health human serum sample. The un-spiked serum was used as blank in this experiment. The recovery ranged from 99% to 110% with an overall mean recovery of 107%.
Sample Values
| Sample Matrix | Sample Evaluated | Range (ng/ml) | Detectable (%) | Mean of Detectable (ng/ml) |
|---|---|---|---|---|
| Serum | 30 | 0.28-24.70 | 100 | 9.93 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of VEGFR2/KDR in this assay. No medical histories were available for the donors.
New Products
