Hydroxyproline (Total Collagen) Assay Kit
Key features and details
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- 反应物种:
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- 靶点名称:
- 偶联标记说明:
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Brand:
CAT.NO. : ARG82320
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Product Details
内含物
| 货号 | 内含物名称 | 包装 | 温度 |
|---|---|---|---|
| ARG82320-001 | 96-well plate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG82320-002 | Collagen standard (1200 µg/ml) | 1 vial | 4°C |
| ARG82320-003 | Assay buffer | 1 vial | 4°C |
| ARG82320-004 | Detection reagent A | 2 vials | 4°C |
| ARG82320-005 | Detection reagent B | 1 vial | 4°C |
| ARG82320-006 | Special screw-capped tubes | 100 ea | Room temperature |
| ARG82320-007 | Plate sealer | 2 strips | Room temperature |
概述
| 产品描述 | ARG82320 Hydroxyproline (Total Collagen) Assay Kit is a detection kit for the quantification of Hydroxyproline in tissue and cell extracts which can be used as a direct measure of the amount of collagen present. |
|---|---|
| 反应物种 | Hu, Ms, Rat, Mamm |
| 应用 | FuncSt |
| 特异性 | The kit is based on the detection of hydroxyproline. Hydroxyproline is a non-proteinogenic amino acid, which in mammals occurs in elastin and collagen. Its presence is mainly limited to the triple helix of collagen, where its presence increases the triple helix stability. Hydroxyproline is formed posttranslationally from specific proline residues by action of the enzyme prolylhydroxylase. Hydroxyproline in tissue hydrolysates can be used as a direct measure of the amount of collagen present. |
| 靶点名称 | Hydroxyproline (Total Collagen) |
| 偶联标记说明 | Read at 570 nm. |
| 灵敏度 | 2.5 µg/ml |
| 样品类型 | Tissue and cell extracts. |
| 标准范围 | 6.25 - 300 µg/ml |
| 样本量 | Tissue: 100 µl Cell extracts: 50 µl |
| 別名 | OI1; OI2; OI3; OI4; EDSC; Collagen alpha-1(I) chain; Alpha-1 type I collagen |
应用说明
| 检测时间 | ~ 1.5hours |
|---|
属性
| 形式 | 96 well |
|---|---|
| 存放说明 | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | COL1A1 |
| 全名 | collagen, type I, alpha 1 |
| 背景介绍 | This gene encodes the pro-alpha1 chains of type I collagen whose triple helix comprises two alpha1 chains and one alpha2 chain. Type I is a fibril-forming collagen found in most connective tissues and is abundant in bone, cornea, dermis and tendon. Mutations in this gene are associated with osteogenesis imperfecta types I-IV, Ehlers-Danlos syndrome type VIIA, Ehlers-Danlos syndrome Classical type, Caffey Disease and idiopathic osteoporosis. Reciprocal translocations between chromosomes 17 and 22, where this gene and the gene for platelet-derived growth factor beta are located, are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans, resulting from unregulated expression of the growth factor. Two transcripts, resulting from the use of alternate polyadenylation signals, have been identified for this gene. [provided by R. Dalgleish, Feb 2008] |
| 生物功能 | Type I collagen is a member of group I collagen (fibrillar forming collagen). [UniProt] |
| 细胞定位 | Secreted, extracellular space, extracellular matrix. [UniProt] |
| 翻译后修饰 | Proline residues at the third position of the tripeptide repeating unit (G-X-P) are hydroxylated in some or all of the chains. Proline residues at the second position of the tripeptide repeating unit (G-P-X) are hydroxylated in some of the chains. O-linked glycan consists of a Glc-Gal disaccharide bound to the oxygen atom of a post-translationally added hydroxyl group. [UniProt] |
检测图片 (1)
ARG82320 Hydroxyproline (Total Collagen) Assay Kit standard curve image
ARG82320 Hydroxyproline (Total Collagen) Assay Kit results of a typical standard run with optical density reading at 570 nm.
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