Insulin Mouse Monoclonal Antibody (2D11-H5)
Key features and details
- Reactivity: Mouse, Rat, Human
- Application: IP, IF, IHC(P), ELISA
- Host: Mouse
- Clonality: Monoclonal
- Isotype: IgG1 kappa light chain
- Target Name: Insulin
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Brand:
CAT.NO. : ARA0021
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Size:
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Product Details
Background
Insulin is a secreted peptide hormone that elicits metabolic effects such as increases in glucose uptake and glycogen synthesis leading to a decrease in blood glucose concentration. Insulin is first formed as a precursor molecule, preproinsulin, which is later cleaved to proinsulin and finally to the mature Insulin hormone. Mature Insulin consists of 51 amino acids, contained within an A chain and a B chain that are connected by two disulfide bridges. It increases cell permeability to monosaccharides, amino acids and fatty acids. Insulin is secreted by the pancreas at basal levels in the absence of exogenous stimuli, with secretion increasing in response to glucose. Insulin action is effected by the binding of Insulin to cell - surface receptors on the target cell membrane. Defects of Insulin are the cause of hyperproinsulinemia and of type 2 diabetes mellitus.
Application
Insulin (2D11 - H5) is recommended for detection of Insulin of mouse, rat and human origin by immunoprecipitation [1 - 2 µg per 100 - 500 µg of total protein (1 ml of cell lysate)], immunofluorescence (starting dilution 1:50, dilution range 1:50 - 1:500), immunohistochemistry (including paraffin - embedded sections) (starting dilution 1:50, dilution range 1:50 - 1:500), and solid phase ELISA (starting dilution 1:30, dilution range 1:30 - 1:3000).
Data
Insulin (2D11 - H5). Immunoperoxidase staining of formalin fixed MIA PaCa - 2 cell smear showing cytoplasmic localization (A, upper). Immunoperoxidase staining of formalin fixed, paraffin - embedded human pancreas tissue showing cytoplasmic staining of Islets of Langerhans (B, below).
Insulin (2D11 - H5). Immunoperoxidase staining of formalin fixed, paraffin - embedded human pancreas tissue showing cytoplasmic staining of Islets of Langerhans.
Sourse
Insulin (2D11 - H5) is a mouse monoclonal antibody raised against amino acids 1 - 84 representing full length Insulin of porcine origin.
Storage
Store at 4°C, DO NOT FREEZE. Stable for one year from the date of shipment.
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