Intracellular ROS Assay Kit (Fluorometric)
CAT.NO. : ARG81192
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概述
反应物种 | All |
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应用 | FuncSt |
靶点名称 | ROS |
偶联标记 | Un-conjugated |
偶联标记说明 | Read the fluorescence with a fluorescence plate reader at 480 nm excitation / 530 nm emission. |
灵敏度 | 0.01 nM |
样品类型 | Cultured cells |
标准范围 | 0.01 - 10000 nM |
样本量 | Cultured cells in either a clear or black 96-well cell culture plate. |
应用说明
检测时间 | ~ 1 hour |
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属性
形式 | Liquid |
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存放说明 | Store components at 4°C or -20°C. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
检测图片 (3)
ARG81192 Intracellular ROS Assay Kit (Fluorometric) standard curve image
ARG81192 Intracellular ROS Assay Kit (Fluorometric) results of a typical standard run with optical density reading at 480 nm excitation / 530 nm emission.
ROS in HeLa cells treated with H2O2
50,000 HeLa cells in a 96-well plate were first pretreated with 1 mM DCFH-DA for 60 minutes at 37°C. Cells were then treated with various concentrations of H2O2 for 20 minutes.
DCF Fluorescence in HeLa cells treated with H2O2
DCF Fluorescence in H2O2 treated HeLa cells after 1 hour. Left: 0 µM; Right: 1000 µM.
