Intracellular ROS Assay Kit (Fluorometric)

Key features and details

  • 反应物种: All
  • 应用: FuncSt
  • 靶点名称: ROS
  • 偶联标记: Un-conjugated
  • 偶联标记说明: Read the fluorescence with a fluorescence plate reader at 480 nm excitation / 530 nm emission.
  • 灵敏度: 0.01 nM
  • 样品类型: Cultured cells
  • 标准范围: 0.01 - 10000 nM
  • 样本量: Cultured cells in either a clear or black 96-well cell culture plate.
  • Brand:
CAT.NO. : ARG81192
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Product Details
概述
反应物种All
应用FuncSt
靶点名称ROS
偶联标记Un-conjugated
偶联标记说明Read the fluorescence with a fluorescence plate reader at 480 nm excitation / 530 nm emission.
灵敏度0.01 nM
样品类型Cultured cells
标准范围0.01 - 10000 nM
样本量Cultured cells in either a clear or black 96-well cell culture plate.
应用说明
检测时间~ 1 hour
属性
形式Liquid
存放说明Store components at 4°C or -20°C. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
注意事项For laboratory research only, not for drug, diagnostic or other use.
检测图片 (3)
  • ARG81192 Intracellular ROS Assay Kit (Fluorometric) standard curve image

    ARG81192 Intracellular ROS Assay Kit (Fluorometric) results of a typical standard run with optical density reading at 480 nm excitation / 530 nm emission.

  • ROS in HeLa cells treated with H2O2

    50,000 HeLa cells in a 96-well plate were first pretreated with 1 mM DCFH-DA for 60 minutes at 37°C. Cells were then treated with various concentrations of H2O2 for 20 minutes.

  • DCF Fluorescence in HeLa cells treated with H2O2

    DCF Fluorescence in H2O2 treated HeLa cells after 1 hour. Left: 0 µM; Right: 1000 µM.

参考文献

TNFAIP3 interacting protein 2 relieves lipopolysaccharide (LPS)-induced inflammatory injury in endometritis by inhibiting NF-kappaB activation

FuncSt / Human

Xinxin Qian et al.
Immun Inflamm Dis.,  (2023)

publication_link

 

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