Irisin ELISA Kit

Key features and details

  • 产品描述: ARG81196 Irisin ELISA Kit is a Competitive Enzyme Immunoassay kit for the quantification of Irisin in serum and plasma.
  • 反应物种: Hu, Ms, Rat, Dog
  • 应用: ELISA
  • 特异性: Irisin (Human, Mouse, Rat, Canine): 100%Irisin (aa. 42-112) (Human, Mouse, Rat, Canine): 100%Irisin, Recombinant (Human, Mouse, Rat, Canine): 100%FNDC5 (Isoform 4) (Human, Mouse, Rat): 9%Irisin precursor, C-terminal / FNDC5 (aa. 165 - 212) (Human) / FNDC5 (aa. 162-209) (Mouse, Rat): 0%Irisin (aa. 42-95) (Human, Mouse, Rat, Canine): 0%
  • 靶点名称: Irisin
  • 偶联标记: HRP
  • 偶联标记说明: Substrate: TMB and read at 450 nm
  • 灵敏度: 6.2 ng/ml
  • 检测范围: Linear Range: 6.2 - 205 ng/ml
  • Brand:
CAT.NO. : ARG81196
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Product Details
概述
产品描述ARG81196 Irisin ELISA Kit is a Competitive Enzyme Immunoassay kit for the quantification of Irisin in serum and plasma.
反应物种Hu, Ms, Rat, Dog
应用ELISA
特异性Irisin (Human, Mouse, Rat, Canine): 100%
Irisin (aa. 42-112) (Human, Mouse, Rat, Canine): 100%
Irisin, Recombinant (Human, Mouse, Rat, Canine): 100%
FNDC5 (Isoform 4) (Human, Mouse, Rat): 9%
Irisin precursor, C-terminal / FNDC5 (aa. 165 - 212) (Human) / FNDC5 (aa. 162-209) (Mouse, Rat): 0%
Irisin (aa. 42-95) (Human, Mouse, Rat, Canine): 0%
靶点名称Irisin
偶联标记HRP
偶联标记说明Substrate: TMB and read at 450 nm
灵敏度6.2 ng/ml
检测范围Linear Range: 6.2 - 205 ng/ml
样品类型Serum and plasma.
标准范围0.1 - 1000 ng/ml
样本量50 µl
精确Intra-Assay CV: less than 10%
Inter-Assay CV: less than 15%
別名irisin; FRCP2; Fibronectin type III domain-containing protein 5; Fibronectin type III repeat-containing protein 2
应用说明
检测时间~ 5 hours
属性
形式96 well
存放说明Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
注意事项For laboratory research only, not for drug, diagnostic or other use.
生物信息
数据库连接

GeneID: 252995 Human FNDC5

GeneID: 260327 Rat FNDC5

GeneID: 384061 Mouse FNDC5

基因名称FNDC5
全名fibronectin type III domain containing 5
背景介绍This gene encodes a secreted protein that is released from muscle cells during exercise. The encoded protein may participate in the development of brown fat. Translation of the precursor protein initiates at a non-AUG start codon at a position that is conserved as an AUG start codon in other organisms. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2013]
生物功能Irisin: Contrary to mouse, may not be involved in the beneficial effects of muscular exercise, nor in the induction of browning of human white adipose tissue. [UniProt]
产品亮点Related products:
Irisin antibodies; Irisin ELISA Kits;
New ELISA data calculation tool:
Simplify the ELISA analysis by GainData
翻译后修饰The extracellular domain is cleaved and released from the cell membrane.
N-Glycosylated.
检测图片 (1)
  • ARG81196 Irisin ELISA Kit standard curve image

    ARG81196 Irisin ELISA Kit results of a typical standard run with optical density reading at 450 nm.

参考文献

Polysaccharides from Enteromorpha prolifera improves insulin sensitivity and promotes adipose thermogenesis in diet-induced obese mice associated with activation of PGC-1α-FNDC5/irisin pathway

ELISA / Mouse / Serum

FeiXie et al.
Journal of Functional Foods,  (2022)

publication_link

 

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Curcumin improves insulin sensitivity and increases energy expenditure in high-fat-diet-induced obese mice associated with activation of FNDC5/irisin

ELISA / Mouse

Tiande Zou et al.
Nutrition.,  (2021)

publication_link

 

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