Langerin / CD207 Rabbit Monoclonal Antibody(ARA823)
Key features and details
- Reactivity:Human
- Application:WB, IHC - P, IF
- Host:Rabbit
- Clonality:Monoclonal
- lsotype:IgG
- Target:Langerin / CD207
-
Brand:
CAT.NO. : ARA6615
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Product Details
Background
Langerin (CD207) is a type II transmembrane protein which is encoded by the CD207 gene in humans. It was discovered by scientists Sem Saeland and Jenny Valladeau as a main part of Birbeck granules. Langerin is C-type lectin receptor on Langerhans cells (LCs) and in mice also on dermal interstitial CD103+ dendritic cells (DC) and on resident CD8+ DC in lymph nodes. Langerin has similar function and structure as a DCs surface protein DC-SIGN (CD209). Both receptors bind similar antigens via the CRD, for instance Mycobacterium tuberculosis and HIV-1. However, whereas HIV-1 binding to langerin leads to the elimination of the virus, HIV-1 binding to DC-SIGN leads to infection of the cell.
Application
|
Applications |
Tested Dilution |
|
WB |
1:1,000 |
|
IHC-P |
1:4,000 |
|
IF-Cell |
1:2,500 |
|
IF-Tissue |
1:200 - 1:1,000 |
Overview
|
Immunogen |
Synthetic peptide within human Langerin aa 1 - 43 / 328. |
|
Species Reactivity |
Human |
|
Validated Applications |
WB, IHC - P, IF - Cell, IF - Tissue |
|
Molecular Weight |
Predicted band size: 37 kDa |
|
Positive Controls |
Human lymph nodes tissue, human skin tissue, human tonsil tissue, 293T cells transfected with CD207/Langerin. |
|
Conjugation |
unconjugated |
|
Form |
Liquid |
|
Concentration |
1μg/μl |
|
Storage Buffer |
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
|
Isotype |
IgG |
|
Purification Method |
Protein A affinity purified. |
Data
Western blot analysis of Langerin / CD207 on different lysates with Rabbit anti-Langerin / CD207 antibody (ARA6615) at 1/1,000 dilution.
Lane 1: 293T-NT cell lysate
Lane 2: 293T-OE-CD207/Langerin cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 37 kDa
Observed band size: 37 kDa
Exposure time: 20 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Immunohistochemical analysis of paraffin-embedded human lymph nodes tissue with Rabbit anti-Langerin / CD207 antibody (ARA6615) at 1/4,000 dilution.
Immunohistochemical analysis of paraffin-embedded human skin tissue with Rabbit anti-Langerin / CD207 antibody (ARA6615) at 1/4,000 dilution.
Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-Langerin / CD207 antibody (ARA6615) at 1/4,000 dilution.
Immunohistochemical analysis of paraffin-embedded human brain tissue (negative) with Rabbit anti-Langerin / CD207 antibody (ARA6615) at 1/2,000 dilution.
Immunocytochemistry analysis of 293T cells transfected with or without CD207/Langerin labeling Langerin / CD207 with Rabbit anti-Langerin / CD207 antibody (ARA6615) at 1/2,500 dilution.
Storage
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
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