Lupin ELISA Kit

Key features and details

  • 产品描述: ARG80804 Lupin ELISA Kit is a Enzyme Immunoassay kit for the quantification of Lupin in food (extraction, dilution).
  • 应用: ELISA
  • 靶点名称: Lupin
  • 偶联标记: HRP
  • 偶联标记说明: Substrate: TMB and read at 450 nm
  • 灵敏度: 0.2 ppm
  • 样品类型: Food (extraction, dilution).
  • 标准范围: 2 - 30 ppm
  • 样本量: 100 µl
  • Brand:
CAT.NO. : ARG80804
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Product Details
概述
产品描述ARG80804 Lupin ELISA Kit is a Enzyme Immunoassay kit for the quantification of Lupin in food (extraction, dilution).
应用ELISA
靶点名称Lupin
偶联标记HRP
偶联标记说明Substrate: TMB and read at 450 nm
灵敏度0.2 ppm
样品类型Food (extraction, dilution).
标准范围2 - 30 ppm
样本量100 µl
应用说明
检测时间20, 20 min (RT), 20 min (RT/dark)
属性
形式96 well
存放说明Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
注意事项For laboratory research only, not for drug, diagnostic or other use.
生物信息
全名Lupin
背景介绍Lupin belongs to the legume family. With about 35-45% the fraction of proteins in lupin seed is very high. Some of these proteins, predominantly those in the alpha, beta and gamma-Conglutin fractions are known for being allergenic, due to cross-reaction to allergens of soy and peanut. Because of its convenient nutritional properties the use of lupin flour as a food ingredient is highly increasing. Lupin is often used in dietary products, especially for milk allergic people, vegetarians and patients with celiac disease. In food production three (sweet) lupin species are commonly used: Lupinus albus, Lupinus angustifolius and Lupinus luteus.

For lupin allergic persons hidden lupin allergens in food are a critical problem. Already very low amounts of lupin can cause allergic reactions, which may lead to anaphylactic shock in severe cases. Because of this, lupin allergic per­sons must strictly avoid the consumption of lupin containing food. Cross-contamination, most­ly in consequence of the production pro­cess, is often noticed. This explains why in many cases the existence of lupin residues in food cannot be excluded. For this reason sensitive detection systems for lupin residues in food­stuffs are required.

The Lupin ELISA represents a highly sensitive detection system for lupin and is particularly capable of the quantification of residues in sausage, bakery products, potato products, ketchup and juices. Due to high cross-reactivity the test is suitable for the detection of all commonly used lupin species.
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检测图片 (1)
  • ARG80804 Lupin ELISA Kit standard curve image

    ARG80804 Lupin ELISA Kit results of a typical standard run with optical density reading at 450nm.

参考文献

Lupine allergens: Clinical relevance, molecular characterization, cross-reactivity, and detection strategies

(Review) / (Review)

Caterina Villa et al.
Compr Rev Food Sci Food Saf.,  (2020)

publication_link

 

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