Mammal VEGF-C ELISA Kit

Protocol Booklet

Key features and details

Specification: 96 Test
Sensitivity: 1.82 pg/ml (50 μl)
Standard Curve Range: 13.72~10000 pg/ml
Standard Curve Gradient: 7 Points/3 Folds
Number of Incubations: 2
Sample Volume: 50 μl
Assay type: Sandwich Elisa
Operation Duration: 120min

Brand:

CAT.NO. : AEH0113

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Size:

96T

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Product Details

Background

Vascular endothelial growth factor C (VEGF-C) is a homodimeric ligand of VEGF R3/Flt-4 and is synthesized with N- and C-terminal propeptides. Fully processed VEGF-C containing only the VEGF homology domain can additionally bind and activate VEGF R2/KDR/Flk-1. VEGF-C interactions with VEGF R3 are critical for lymphangiogenesis. Both the ligand and receptor are usually co-expressed at sites of lymphatic vessel sprouting, in the embryo, and in various pathological conditions. Over-expression of VEGF-C in tumor cells induces tumoral lymphatic hyperplasia, neoangiogenesis, and vessel sprouting, resulting in enhanced lymph flow and metastasis to regional lymph nodes.

typical data

pg/ml	O.D.		Average	Corrected
0.00	0.0193	0.0193	0.0193
13.72	0.0278	0.0271	0.0275	0.0082
41.15	0.0444	0.0434	0.0439	0.0246
123.46	0.0984	0.0931	0.0958	0.0765
370.37	0.2400	0.2467	0.2434	0.2241
1111.11	0.6659	0.6791	0.6725	0.6532
3333.33	1.7940	1.8210	1.8075	1.7882
10000.00	3.8780	3.7850	3.8315	3.8122

precision

	Intra-assay Precision			Inter-assay Precision
Sample Number	S1	S2	S3	S1	S2	S3
Sample Number	22	22	22	6	6	6
Average（pg/ml）	240.1	1112.3	3324.8	233.3	1079.7	3258.5
Standard deviation	12.3	50.9	159.1	7.2	37.5	71.2
Coefficient of variation（%）	5.1	4.6	4.8	3.1	3.5	2.2

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.

spike recovery

The spike recovery was evaluated by spiking 3 levels of mammal VEGF-C into health serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 80% to 110% with an overall mean recovery of 91%.

sample values

Sample Matrix

Sample

Evaluated

Range

(pg/ml)

Detectable

Mean of Detectable

(pg/ml)

Serum

474.36-2997.14

100

1487.71

Serum/Plasma - Thirty samples from apparently healthy volunteers were evaluated for the presence of mammal VEGF-C in this assay. No medical histories were available for the donors.

Sample Matrix

Sample

Evaluated

Range

(pg/ml)

Detectable

Mean of Detectable

(pg/ml)

Serum

24.68-42.41

100

33.72

Serum/Plasma – Thirty samples from apparently healthy mice were evaluated for the presence of mammal VEGF-C in this assay. No medical histories were available for the donors.

Sample Matrix

Sample

Evaluated

Range

(pg/ml)

Detectable

Mean of Detectable

(pg/ml)

Serum

12.19-175.72

100

64.70

Serum/Plasma – Thirty samples from apparently healthy rats were evaluated for the presence of mammal VEGF-C in this assay. No medical histories were available for the donors.

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