Mast Cell Tryptase Rabbit Monoclonal Antibody(ARA833)
Key features and details
- Reactivity:Human, Mouse
- Application:WB, IP,IHC
- Host: Rabbit
- Clonality:Monoclonal
- Target:Mast Cell Tryptase
-
Brand:
CAT.NO. : ARA6647
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US＄ Please choose
Size:
Trail, Bulk size or Custom requests Please contact us
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Product Details
Background
Mast cells are connective tissue cells derived from blood-forming tissues that line arterial walls and secrete substances, which mediate inflammatory and immune responses. Mast cell chymase, known as CMA1, is a major secreted serine protease that is involved in vasoactive peptide generation, extracellular matrix degradation and regulation of gland secretion. The human chymase gene, which maps to human chromosome 14q11.2, encodes a preproenzyme with a 19-amino acid signal peptide, an acidic 2-amino acid propeptide and a 226-amino acid catalytic domain. Tryptases comprise a family of trypsin-like serine proteases that are enzymatically active as heparin-stabilized tetramers. There are four functional genes for tryptase: α I, β I, β II and γ I, which map to human chromosome 16p13.3, with β tryptases representing the main isoenzymes expressed in mast cells. Mast cell proteases are a family of rodent protein homologs to human tryptases that are specifically expressed in mast cells and may serve as highly specific markers in the analysis of mast cell heterogeneity, differentiation and function.
Application
|
Application |
Dilution Ratio |
|
WB |
1:1,000 |
|
IHC |
1:5,000-1:10,000 |
|
IP |
Use at an assay dependent concentration. |
|
mIHC |
1:3,000-1:5,000 |
Overview
|
Product Name |
Mast Cell Tryptase Recombinant Rabbit Monoclonal Antibody |
|
Antibody Type |
Recombinant Rabbit monoclonal Antibody |
|
Immunogen |
Recombinant protein within Human Mast Cell Tryptase aa 10-275 /275. |
|
Species Reactivity |
Human, Mouse |
|
Validated Applications |
WB, IHC-P, IP, mIHC |
|
Molecular Weight |
Predicted band size: 30 kDa |
|
Positive Control |
Human lung tissue lysates, mouse skin tissue lysates, human lung tissue, human tonsil tissue, human small intestine tissue, human prostate carcinoma tissue. |
|
Conjugation |
unconjugated |
Data
Immunohistochemical analysis of paraffin-embedded human lung tissue with Rabbit anti-Mast Cell Tryptase antibody (ARA6647) at 1/10,000 dilution.
Western blot analysis of Mast Cell Tryptase on different lysates with Rabbit anti-Mast Cell Tryptase antibody (ARA6647) at 1/1,000 dilution.
Lane 1: Human lung tissue lysate (40 µg/Lane)
Lane 2: Mouse skin tissue lysate (40 µg/Lane)
Predicted band size: 30 kDa Observed band size: 35 kDa Exposure time: 59 seconds; ECL: K1801; 4-20% SDS-PAGE gel.
Storage
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
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