MCU Mouse Monoclonal Antibody(ARA997)

Key features and details

Reactivity:Human, Mouse, Rat, Monkey
Application:WB, IHC, IF/ICC
Host:Mouse
Clonality:Monoclonal
lsotype: IgM
Target:MCU

Brand:

CAT.NO. : ARA6902

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Size:

50μL 100μL

Trail, Bulk size or Custom requests Please contact us

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Product Details

Background

Mitochondrial inner membrane calcium uniporter that mediates calcium uptake into mitochondria. Constitutes the pore-forming and calcium-conducting subunit of the uniporter complex (uniplex). Activity is regulated by MICU1 and MICU2. At low Ca(2+) levels MCU activity is down-regulated by MICU1 and MICU2; at higher Ca(2+) levels MICU1 increases MCU activity. Mitochondrial calcium homeostasis plays key roles in cellular physiology and regulates cell bioenergetics, cytoplasmic calcium signals and activation of cell death pathways. Involved in buffering the amplitude of systolic calcium rises in cardiomyocytes. While dispensable for baseline homeostatic cardiac function, acts as a key regulator of short-term mitochondrial calcium loading underlying a 'fight-or-flight' response during acute stress: acts by mediating a rapid increase of mitochondrial calcium in pacemaker cells. participates in mitochondrial permeability transition during ischemia-reperfusion injury. Regulates glucose-dependent insulin secretion in pancreatic beta-cells by regulating mitochondrial calcium uptake. Mitochondrial calcium uptake in skeletal muscle cells is involved in muscle size in adults. Regulates synaptic vesicle endocytosis kinetics in central nerve terminal. Involved in antigen processing and presentation.

Application

Application	Dilution Ratio
WB	1:1000 - 1:5000
IHC	1:20 - 1:50
ICC/IF	1:20 - 1:50

Overview

Antibody Type	Mouse Monoclonal Antibody
Immunogen	Recombinant protein within Human MCU aa 51-325 / 351.
Species Reactivity	Human, Mouse, Rat, Monkey
Validated Applications	WB, IHC-P, IF-Cell
Molecular Weight	Predicted band size: 40 kDa
Positive Control	HCT 116 cell lysate, PANC-1 cell lysate, A549 cell lysate, Hela cell lysate, COS-1 cell lysate, NIH/3T3 cell lysate, C2C12 cell lysate, C6 cell lysate, PC-12 cell lysate, Mouse colon tissue lysate, Mouse testis tissue lysate, Rat colon tissue lysate, Rat heart tissue lysate, human colon tissue, human colon cancer tissue, HCT 116, NIH/3T3, C6.
Conjugation	unconjugated
RRID	AB_3068930
Form	Liquid
Storage Buffer	PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype	IgM
Purification Method	Protein A affinity purified.

Data

Western blot analysis of MCU on different lysates with MCU Mouse Monoclonal Antibody(ARA997).

Immunohistochemical analysis of paraffin-embedded human colon tissue with MCU Mouse Monoclonal Antibody(ARA997).

Immunocytochemistry analysis of C6 cells labeling MCU with MCU Mouse Monoclonal Antibody(ARA997).

Storage

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Research Use Only

For Research Use Only. Not for use in diagnostic procedures.

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