MDM2 Rabbit Monoclonal Antibody(ARB985)
Key features and details
- Target:
- Clone ID:
- Host:
- Molecular Weight:
- Purity:
- Species Cross-reactivity:
- Applications:
- Swissprot ID:
-
Brand:
CAT.NO. : ARB6777
RMB Please choose
RMB Please choose
Size:
Trail, Bulk size or Custom requests Please contact us
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Product Details
Background
The MDM2 (murine double minute-2) protein was initially identified as an oncogene in a murine transformation system. MDM2 functions to bind p53 and block p53-mediated transactivation of cotransfected reporter constructs. p53 is the most commonly mutated gene in human cancer identified to date. Expression of p53 leads to inhibition of cell growth by preventing progression of cells from G1 to S phase of the cell cycle. Most importantly, p53 functions to cause arrest of cells in the G1 phase of the cell cycle following any exposure of cells to DNA-damaging agents. The MDM2 gene is amplified in a high percentage of human sarcomas that retain wt p53 and tumor cells that overexpress MDM2 can tolerate high levels of p53 expression. These findings argue that MDM2 overexpression represents at least one mechanism by which p53 function can be abrogated during tumorigenesis.
Application
|
Application |
Dilution Ratio |
|
IHC |
1:100 - 1:200 |
Overview
|
Predicted Molecular Wt |
55kDa |
|
Species Cross-reactivity |
Human |
|
Applications |
IHC-P |
|
Purity |
ProA affinity purified IgG |
|
Form |
Liquid |
|
Swissprot ID |
Q00987 |
|
Subcellular location |
Nuclear |
|
Recommended method |
Heat induced epitope retrieval with Tris-EDTA buffer (pH 9.0), primary antibody incubate at RT (18℃-25℃) for 30 minutes |
|
Immunogen |
Synthetic peptide within Human MDM2. |
|
Storage Buffer |
PBS 59%, Sodium azide 0.01%, Glycerol 40%, BSA 0.05% |
Data
Immunohistochemical staining of human dedifferentiated liposarcoma tissue using MDM2 Rabbit Monoclonal Antibody(ARB985)
Storage
Store at -20°C. Stable for one year from the date of shipment.
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
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