MLH1 Rabbit Monoclonal Antibody(ARA726)

Key features and details

Reactivity:Human
Application:WB, IHC
Host:Rabbit
Clonality:Monoclonal
Target:MLH1

Brand:

CAT.NO. : ARA6513

RMB Please choose

Size:

1mL 100μL

Trail, Bulk size or Custom requests Please contact us

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Product Details

Background

MutL Homolog 1 (MLH1) is a protein involved in the mismatch-repair pathway. This protein is commonly associated with hereditary non-polyposis colorectal cancer, as the MLH1 gene is frequently mutated in patients with this cancer. Studies have shown MLH1 to be deficient in a high percentage of patients with microsatellite instability, as well as endometrial and ovarian cancers. Use of Anti-MLH1 is optimized when paired in an IHC panel with MSH6, MSH2, and PMS2. Anti-MLH1 is useful in the detection of MLH1 in a number of normal and neoplastic tissues, and for identifying a loss of MLH1 in tumours that are microsatellite-unstable.

Application

Application	Dilution Ratio
WB	1:200-1:2000
IHC-P	1:200-1:1000

Overview

Antibody Type	Recombinant Rabbit monoclonal Antibody
Immunogen	Synthetic peptide corresponding to Human MLH1 aa 700-756.
Species Reactivity	Human, Mouse, Rat
Validated Applications	WB, IHC-P
Molecular Weight	Predicted band size: 85 kDa
Positive Control	HeLa cell lysate, HCT 116 cell lysate, A549 cell lysate, C2C12 cell lysate, NIH/3T3 cell lysate, human appendix tissue, human colon tissue, human B-cell lymphoblastic lymphoma tissue, human NKT cell lymphoma tissue, mouse testis tissue, mouse thymus tissue, human colon carcinoma tissue, rat small intestine tissue.
Conjugation	unconjugated
RRID	AB_3072442
Form	Liquid
Storage Buffer	PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype	IgG
Purification Method	Protein A affinity purified.