MLH1 Rabbit Monoclonal Antibody(ARA749)
Key features and details
- Target:
- Clone ID:
- Host:
- Clone Type:
- Purity:
- Species Cross-reactivity:
- Applications:
- Isotype:
-
Brand:
CAT.NO. : ARA6536
RMB Please choose
RMB Please choose
Size:
Trail, Bulk size or Custom requests Please contact us
Product Details
Background
DNA repair is initiated by MutS alpha (MSH2-MSH6) or MutS beta (MSH2-MSH6) binding to a dsDNA mismatch, then MutL alpha is recruited to the heteroduplex. It introduces single-strand breaks near the mismatch and thus generates new entry points for the exonuclease EXO1 to degrade the strand containing the mismatch. DNA methylation would prevent cleavage and therefore assure that only the newly mutated DNA strand is going to be corrected. MutL alpha (MLH1-PMS2) interacts physically with the clamp loader subunits of DNA polymerase III, suggesting that it may play a role to recruit the DNA polymerase III to the site of the MMR.
Application
|
Application |
Dilution Ratio |
|
IHC-P |
Not specified |
|
FC |
1:20-1:100 |
|
IF/ICC |
1:200-1:1,000 |
Overview
|
Host Species |
Rabbit |
|
Species Cross-reactivity |
Human |
|
Application |
IHC-P, FC, IF/ICC |
|
Immunogen |
A synthetic peptide corresponding to MLH1 residues within aa400-500 of MLH1 was used as an immunogen. |
|
Purity |
ProA affinity purified IgG |
|
Clone Type |
Recombinant Monoclonal |
|
Isotype |
IgG |
|
Buffer |
PBS 59%, Sodium azide 0.01%, Glycerol 40%, BSA 0.05% |
Storage
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
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