Mouse ACE2 ELISA Kit
Key features and details
- 产品描述:
- 反应物种:
- 应用:
- 特异性:
- 靶点名称:
- 偶联标记:
- 偶联标记说明:
- 灵敏度:
- 样品类型:
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Brand:
CAT.NO. : ARG81839
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Product Details
内含物
| 货号 | 内含物名称 | 包装 | 温度 |
|---|---|---|---|
| ARG81839-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81839-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG81839-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81839-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81839-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81839-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81839-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81839-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81839-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81839-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81839-011 | Plate sealer | 4 strips | Room temperature |
概述
| 产品描述 | ARG81839 Mouse ACE2 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Mouse ACE2 in serum, plasma (heparin) and cell culture supernatants. |
|---|---|
| 反应物种 | Ms |
| 应用 | ELISA |
| 特异性 | There is no detectable cross-reactivity with other relevant proteins. |
| 靶点名称 | ACE2 |
| 偶联标记 | HRP |
| 偶联标记说明 | Substrate: TMB and read at 450 nm. |
| 灵敏度 | 31.25 pg/ml |
| 样品类型 | Serum, plasma (heparin) and cell culture supernatants. |
| 标准范围 | 62.5 - 4000 pg/ml |
| 样本量 | 100 µl |
| 精确 | Intra-Assay CV: 6.2%; Inter-Assay CV: 6.9% |
| 別名 | Angiotensin-converting enzyme homolog; ACEH; Angiotensin-converting enzyme 2; Metalloprotease MPROT15; ACE-related carboxypeptidase; EC 3.4.17.23 |
应用说明
| 检测时间 | ~ 5 hours |
|---|
属性
| 形式 | 96 well |
|---|---|
| 存放说明 | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | ACE2 |
| 全名 | angiotensin I converting enzyme 2 |
| 背景介绍 | The protein encoded by this gene belongs to the angiotensin-converting enzyme family of dipeptidyl carboxydipeptidases and has considerable homology to human angiotensin 1 converting enzyme. This secreted protein catalyzes the cleavage of angiotensin I into angiotensin 1-9, and angiotensin II into the vasodilator angiotensin 1-7. The organ- and cell-specific expression of this gene suggests that it may play a role in the regulation of cardiovascular and renal function, as well as fertility. In addition, the encoded protein is a functional receptor for the spike glycoprotein of the human coronaviruses SARS and HCoV-NL63. [provided by RefSeq, Jul 2008] |
| 生物功能 | Carboxypeptidase which converts angiotensin I to angiotensin 1-9, a peptide of unknown function, and angiotensin II to angiotensin 1-7, a vasodilator. Also able to hydrolyze apelin-13 and dynorphin-13 with high efficiency. May be an important regulator of heart function. In case of human coronaviruses SARS and HCoV-NL63 infections, serve as functional receptor for the spike glycoprotein of both coronaviruses. [UniProt] |
| 产品亮点 | Related products: ACE2 antibodies; ACE2 ELISA Kits; ACE2 recombinant proteins; Related news: HMGB1, a biomarker and therapeutic target in COVID-19 ACE2, receptor of 2019-nCoV New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| 翻译后修饰 | N-glycosylation on Asn-90 may limit SARS infectivity. Proteolytic cleavage by ADAM17 generates a secreted form. Also cleaved by serine proteases: TMPRSS2, TMPRSS11D and HPN/TMPRSS1. [UniProt] |
检测图片 (1)
ARG81839 Mouse ACE2 ELISA Kit standard curve image
ARG81839 Mouse ACE2 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
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