Mouse ADAM12 ELISA Kit
Key features and details
- 产品描述:
- 反应物种:
- 应用:
- 特异性:
- 靶点名称:
- 偶联标记:
- 偶联标记说明:
- 灵敏度:
- 样品类型:
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Brand:
CAT.NO. : ARG81873
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Product Details
内含物
| 货号 | 内含物名称 | 包装 | 温度 |
|---|---|---|---|
| ARG81873-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81873-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG81873-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81873-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81873-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81873-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81873-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81873-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81873-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81873-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81873-011 | Plate sealer | 4 strips | Room temperature |
概述
| 产品描述 | ARG81873 Mouse ADAM12 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Mouse ADAM12 in serum, plasma (heparin) and cell culture supernatants. |
|---|---|
| 反应物种 | Ms |
| 应用 | ELISA |
| 特异性 | There is no detectable cross-reactivity with other relevant proteins. |
| 靶点名称 | ADAM12 |
| 偶联标记 | HRP |
| 偶联标记说明 | Substrate: TMB and read at 450 nm. |
| 灵敏度 | 78 pg/ml |
| 样品类型 | Serum, plasma (heparin) and cell culture supernatants. |
| 标准范围 | 156 - 10000 pg/ml |
| 样本量 | 100 µl |
| 精确 | Intra-Assay CV: 6.2%; Inter-Assay CV: 8.1% |
| 別名 | MCMP; ADAM 12; MCMPMltna; EC 3.4.24.-; Disintegrin and metalloproteinase domain-containing protein 12; Meltrin-alpha; MLTN; MLTNA; CAR10; ADAM12-OT1 |
应用说明
| 检测时间 | ~ 5 hours |
|---|
属性
| 形式 | 96 well |
|---|---|
| 存放说明 | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | Swiss-port # Q61824 Mouse Disintegrin and metalloproteinase domain-containing protein 12 |
|---|---|
| 基因名称 | ADAM12 |
| 全名 | ADAM metallopeptidase domain 12 |
| 背景介绍 | This gene encodes a member of a family of proteins that are structurally related to snake venom disintegrins and have been implicated in a variety of biological processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. Expression of this gene has been used as a maternal serum marker for pre-natal development. Alternative splicing results in multiple transcript variants encoding different isoforms. Shorter isoforms are secreted, while longer isoforms are membrane-bound form. [provided by RefSeq, Jan 2014] |
| 生物功能 | Involved in skeletal muscle regeneration, specifically at the onset of cell fusion. Also involved in macrophage-derived giant cells (MGC) and osteoclast formation from mononuclear precursors (By similarity). [UniProt] |
| 产品亮点 | Related products: ADAM antibodies; ADAM ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| 翻译后修饰 | The precursor is cleaved by a furin endopeptidase. [UniProt] |
检测图片 (1)
ARG81873 Mouse ADAM12 ELISA Kit standard curve image
ARG81873 Mouse ADAM12 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
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