Mouse Angiopoietin 2 ELISA Kit
Key features and details
- 产品描述:
- 反应物种:
- 应用:
- 特异性:
- 靶点名称:
- 偶联标记:
- 偶联标记说明:
- 灵敏度:
- 样品类型:
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Brand:
CAT.NO. : ARG81783
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Product Details
内含物
| 货号 | 内含物名称 | 包装 | 温度 |
|---|---|---|---|
| ARG81783-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81783-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG81783-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81783-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81783-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81783-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81783-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81783-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81783-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81783-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81783-011 | Plate sealer | 4 strips | Room temperature |
概述
| 产品描述 | ARG81783 Mouse Angiopoietin 2 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Mouse Angiopoietin 2 in serum, plasma (heparin, EDTA) and cell culture supernatants. |
|---|---|
| 反应物种 | Ms |
| 应用 | ELISA |
| 特异性 | There is no detectable cross-reactivity with other relevant proteins. |
| 靶点名称 | Angiopoietin 2 |
| 偶联标记 | HRP |
| 偶联标记说明 | Substrate: TMB and read at 450 nm. |
| 灵敏度 | 78 pg/ml |
| 样品类型 | Serum, plasma (heparin, EDTA) and cell culture supernatants. |
| 标准范围 | 156 - 10000 pg/ml |
| 样本量 | 100 µl |
| 精确 | Intra-Assay CV: 5.1%; Inter-Assay CV: 5.3% |
| 別名 | ANG-2; Angiopoietin-2; ANG2; AGPT2 |
应用说明
| 检测时间 | ~ 5 hours |
|---|
属性
| 形式 | 96 well |
|---|---|
| 存放说明 | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | ANGPT2 |
| 全名 | angiopoietin 2 |
| 背景介绍 | The protein encoded by this gene is an antagonist of angiopoietin 1 (ANGPT1) and endothelial TEK tyrosine kinase (TIE-2, TEK). The encoded protein disrupts the vascular remodeling ability of ANGPT1 and may induce endothelial cell apoptosis. Three transcript variants encoding three different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| 生物功能 | Binds to TEK/TIE2, competing for the ANGPT1 binding site, and modulating ANGPT1 signaling. Can induce tyrosine phosphorylation of TEK/TIE2 in the absence of ANGPT1. In the absence of angiogenic inducers, such as VEGF, ANGPT2-mediated loosening of cell-matrix contacts may induce endothelial cell apoptosis with consequent vascular regression. In concert with VEGF, it may facilitate endothelial cell migration and proliferation, thus serving as a permissive angiogenic signal. [UniProt] |
| 产品亮点 | Related products: Angiopoietin antibodies; Angiopoietin ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
检测图片 (1)
ARG81783 Mouse Angiopoietin 2 ELISA Kit standard curve image
ARG81783 Mouse Angiopoietin 2 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
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