Mouse anti-Human Kappa Light Chain antibody [A8B5] (FITC)

Key features and details

  • 产品描述: FITC-conjugated Mouse Monoclonal antibody [A8B5] recognizes Human Kappa Light Chain
  • 反应物种: Hu
  • 不反应物种: Goat, Gpig, Hm, Rb, Sheep
  • 应用: FACS
  • 特异性: The clone A8B5 reacts with kappa light chains (22.5 kDa) of immunoglobulins.
  • 宿主: Mouse
  • 克隆: Monoclonal
  • 克隆号: A8B5
  • 同位型: IgG1
  • Brand:
CAT.NO. : ARG63047
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Product Details
概述
产品描述FITC-conjugated Mouse Monoclonal antibody [A8B5] recognizes Human Kappa Light Chain
反应物种Hu
不反应物种Goat, Gpig, Hm, Rb, Sheep
应用FACS
特异性The clone A8B5 reacts with kappa light chains (22.5 kDa) of immunoglobulins.
宿主Mouse
克隆Monoclonal
克隆号A8B5
同位型IgG1
靶点名称Kappa Light Chain
偶联标记FITC
应用说明
应用建议
应用推荐稀释比
FACS20 µl / 10^6 cells
应用说明* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
属性
形式Liquid
纯化说明The purified antibody is conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC and adjusted for direct use. No reconstitution is necessary.
缓冲液PBS, 15 mM Sodium azide and 0.2% (w/v) high-grade protease free BSA
抗菌剂15 mM Sodium azide
稳定剂0.2% (w/v) high-grade protease free BSA
存放说明Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项For laboratory research only, not for drug, diagnostic or other use.
生物信息
数据库连接

GeneID: 3514 Human IGKC

背景介绍Immunoglobulin classes share the same basic four polypeptide chain structure of two heavy chains (five heavy chains types) and two light chains (kappa, lambda; both having a molecular weight of 22.5kDa). Kappa and lambda consist of a variable region and a constant region and can easily be differentiated by the antigenic properties of the constant region. The ratio of kappa to lambda is 70:30.
研究领域Immune System antibody
检测图片 (4)
  • ARG63047 Mouse anti-Human Kappa Light Chain antibody [A8B5] (FITC) FACS image

    Flow Cytometry: B lymphocytes (CD19+) stained with ARG63047 Mouse anti-Human Kappa Light Chain antibody [A8B5] (FITC).

  • ARG63047 Mouse anti-Human Kappa Light Chain antibody [A8B5] (FITC) FACS image

    Flow Cytometry: Human peripheral whole blood stained with ARG63047 Mouse anti-Human Kappa Light Chain antibody [A8B5] (FITC) (20 µl reagent / 100 µl of peripheral whole blood).

  • ARG63047 Mouse anti-Human Kappa Light Chain antibody [A8B5] (FITC) FACS image

    Flow Cytometry: Human lymphocytes stained with ARG63047 Mouse anti-Human Kappa Light Chain antibody [A8B5] (FITC) (20 µl reagent / 100 µl of peripheral whole blood) and ARG53782 anti-CD19 antibody [LT19] (APC) (10 µl reagent / 100 µl of peripheral whole blood).

  • ARG63047 Mouse anti-Human Kappa Light Chain antibody [A8B5] (FITC) FACS image

    Flow Cytometry: Separation of human Ig kappa light chain positive B-lymphocytes (red-filled) from Ig kappa light chain negative CD19 negative lymphocytes (black-dashed). Human peripheral whole blood stained with ARG63047 Mouse anti-Human Kappa Light Chain antibody [A8B5] (FITC) at (20 µl reagent / 100 µl of peripheral whole blood).

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