Mouse anti-Human Kappa Light Chain antibody [MEM-09]

Key features and details

  • 产品描述: Mouse Monoclonal antibody [MEM-09] recognizes Human Kappa Light Chain
  • 反应物种: Hu
  • 应用: ELISA, FACS, ICC/IF, IHC-P, IP, WB
  • 特异性: The clone MEM-09 reacts with both secreted and B cell-surface human immunoglobulin, specifically reacts with kappa light chains (22.5 kDa). Material immunoprecipitated from human serum with the antibody MEM-09 consists of IgG and traces of IgM.
  • 宿主: Mouse
  • 克隆: Monoclonal
  • 克隆号: MEM-09
  • 同位型: IgG1
  • 靶点名称: Kappa Light Chain
  • Brand:
CAT.NO. : ARG63045
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Product Details
概述
产品描述Mouse Monoclonal antibody [MEM-09] recognizes Human Kappa Light Chain
反应物种Hu
应用ELISA, FACS, ICC/IF, IHC-P, IP, WB
特异性The clone MEM-09 reacts with both secreted and B cell-surface human immunoglobulin, specifically reacts with kappa light chains (22.5 kDa).
Material immunoprecipitated from human serum with the antibody MEM-09 consists of IgG and traces of IgM.
宿主Mouse
克隆Monoclonal
克隆号MEM-09
同位型IgG1
靶点名称Kappa Light Chain
抗原Crude thymus membrane fraction.
偶联标记Un-conjugated
应用说明
应用建议
应用推荐稀释比
ELISAAssay-dependent
FACS5 µg/ml
ICC/IFAssay-dependent
IHC-PAssay-dependent
IPAssay-dependent
WBAssay-dependent
应用说明* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
属性
形式Liquid
纯化Purified from ascites by protein-A affinity chromatography.
纯度> 95% (by SDS-PAGE)
缓冲液PBS (pH 7.4) and 15 mM Sodium azide
抗菌剂15 mM Sodium azide
浓度1 mg/ml
存放说明For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项For laboratory research only, not for drug, diagnostic or other use.
生物信息
数据库连接

GeneID: 3514 Human IGKC

背景介绍Immunoglobulin classes share the same basic four polypeptide chain structure of two heavy chains (five heavy chains types) and two light chains (kappa, lambda; both having a molecular weight of 22.5kDa). Kappa and lambda consist of a variable region and a constant region and can easily be differentiated by the antigenic properties of the constant region. The ratio of kappa to lambda is 70:30.
研究领域Immune System antibody
检测图片 (1)
  • ARG63045 Mouse anti-Human Kappa Light Chain antibody [MEM-09] WB image

    Western blot: Human blood serum samples of different healthy donors stained with ARG63045 Mouse anti-Human Kappa Light Chain antibody [MEM-09].

参考文献

克隆号文献

Cell line-specific control of recombinant monoclonal antibody production by CHO cells.

WB / Chinese Hamster

O'Callaghan PM et al.
Biotechnol Bioeng.,  (2010)

publication_link

 

hr_line

Monoclonal antibodies against human leucocyte antigens. I. Antibodies against beta-2-microglobulin, immunoglobulin kappa light chains, HLA-DR-like antigens, T8 antigen, T1 antigen, a monocyte antigen, and a pan-leucocyte antigen.

Horejsí V et al.
Folia Biol (Praha).,  (1986)

publication_link

 

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