Mouse beta NGF ELISA Kit
Key features and details
- 产品描述:
- 反应物种:
- 应用:
- 特异性:
- 靶点名称:
- 偶联标记:
- 偶联标记说明:
- 灵敏度:
- 样品类型:
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Brand:
CAT.NO. : ARG81663
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Product Details
内含物
| 货号 | 内含物名称 | 包装 | 温度 |
|---|---|---|---|
| ARG81663-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81663-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG81663-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81663-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81663-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81663-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81663-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81663-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81663-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81663-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81663-011 | Plate sealer | 4 strips | Room temperature |
概述
| 产品描述 | ARG81663 Mouse beta NGF ELISA Kit is an Enzyme Immunoassay kit for the quantification of Mouse beta NGF in serum, plasma (heparin, EDTA, citrate) and cell culture supernatants. |
|---|---|
| 反应物种 | Ms |
| 应用 | ELISA |
| 特异性 | There is no detectable cross-reactivity with BDGF, GDNF, CNTF, NT3 and NT4. |
| 靶点名称 | beta NGF |
| 偶联标记 | HRP |
| 偶联标记说明 | Substrate: TMB and read at 450 nm. |
| 灵敏度 | 15.6 pg/ml |
| 样品类型 | Serum, plasma (heparin, EDTA, citrate) and cell culture supernatants. |
| 标准范围 | 31.2 - 2000 pg/ml |
| 样本量 | 100 µl |
| 精确 | Intra-Assay CV: 7.2%; Inter-Assay CV: 7.4% |
| 別名 | HSAN5; Beta-nerve growth factor; Beta-NGF; NGFB |
应用说明
| 检测时间 | ~ 5 hours |
|---|
属性
| 形式 | 96 well |
|---|---|
| 存放说明 | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | NGF |
| 全名 | nerve growth factor (beta polypeptide) |
| 背景介绍 | This gene is a member of the NGF-beta family and encodes a secreted protein which homodimerizes and is incorporated into a larger complex. This protein has nerve growth stimulating activity and the complex is involved in the regulation of growth and the differentiation of sympathetic and certain sensory neurons. Mutations in this gene have been associated with hereditary sensory and autonomic neuropathy, type 5 (HSAN5), and dysregulation of this gene's expression is associated with allergic rhinitis. [provided by RefSeq, Jul 2008] |
| 生物功能 | Nerve growth factor is important for the development and maintenance of the sympathetic and sensory nervous systems. Extracellular ligand for the NTRK1 and NGFR receptors, activates cellular signaling cascades through those receptor tyrosine kinase to regulate neuronal proliferation, differentiation and survival. Inhibits metalloproteinase dependent proteolysis of platelet glycoprotein VI. [UniProt] |
| 产品亮点 | Related products: beta NGF antibodies; beta NGF ELISA Kits; beta NGF recombinant proteins; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
检测图片 (1)
ARG81663 Mouse beta NGF ELISA Kit standard curve image
ARG81663 Mouse beta NGF ELISA Kit results of a typical standard run with optical density reading at 450 nm.
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