Mouse CCL3/MIP-1 alpha ELISA Kit
Protocol Booklet
Key features and details
- Specification:
- Sensitivity:
- Standard Curve Range:
- Standard Curve Gradient:
- Number of Incubations:
- Sample Volume:
- Assay type:
- Operation Duration:
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Brand:
CAT.NO. : AEM0031
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Product Details
Background
MIP-1 alpha (macrophage inflammatory protein 1 alpha) is a member of the CC or beta chemokine subfamily that was originally purified from the conditioned media of an LPS-stimulated murine macrophage cell line. MIP-1 alpha acts as a chemoattractant to a variety of cells including monocytes, T cells, B cells and eosinophils.
- The two human MIP-1 alpha genes arise by duplication/mutation. They code for MIP-1 alpha isoforms CCL3/LD78a and CCL3L1/LD78b, which share 94% amino acid sequence homology. Whereas the human CCL3/LD78a is a single-copy gene, the human CCL3L1/LD78b gene copy number varies within the population. Human CCL3L1/LD78b binds and signals through chemokine receptors CCR1, CCR5. When compared to CCL3/LD78a, CCL3L1/LD78b has higher binding affinity to CCR5, which also functions as a coreceptor for HIV-1 entry. The copy number of CCL3L1 is one of several genetic determinants of HIV-1 susceptibility.
Typical data
| pg/ml | O.D. | Average | Corrected | |
| 0.00 | 0.0159 | 0.0141 | 0.0150 | |
| 1.37 | 0.0232 | 0.0210 | 0.0221 | 0.0071 |
| 4.12 | 0.0341 | 0.0302 | 0.0322 | 0.0172 |
| 12.35 | 0.0651 | 0.0658 | 0.0655 | 0.0505 |
| 37.04 | 0.1703 | 0.1707 | 0.1705 | 0.1555 |
| 111.11 | 0.4507 | 0.4318 | 0.4413 | 0.4263 |
| 333.33 | 1.2730 | 1.2200 | 1.2465 | 1.2315 |
| 1000.00 | 3.1870 | 3.2800 | 3.2335 | 3.2185 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average(pg/ml) | 18.8 | 88.6 | 247.5 | 18.5 | 96.2 | 282.2 |
| Standard Deviation | 1.4 | 3.9 | 13.2 | 1.2 | 6.7 | 10.1 |
| Coefficient of Variation(%) | 7.3 | 4.4 | 5.3 | 6.6 | 6.9 | 3.6 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of mouse CCL3 into health mouse serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 106% to 139% with an overall mean recovery of 128%.
The recovery ranged from 106% to 139% with an overall mean recovery of 128%.
Sample Values
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
| Serum | 30 | 1.2-20.8 | 100 | 4.19 |
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