Mouse CD127 / IL7R ELISA Kit
Key features and details
- 产品描述:
- 反应物种:
- 应用:
- 特异性:
- 靶点名称:
- 偶联标记:
- 偶联标记说明:
- 灵敏度:
- 样品类型:
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Brand:
CAT.NO. : ARG81723
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Product Details
内含物
| 货号 | 内含物名称 | 包装 | 温度 |
|---|---|---|---|
| ARG81723-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81723-002 | Standard | 2 X 20 ng/vial | 4°C |
| ARG81723-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81723-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81723-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81723-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81723-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81723-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81723-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81723-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81723-011 | Plate sealer | 4 strips | Room temperature |
概述
| 产品描述 | ARG81723 Mouse CD127 / IL7R ELISA Kit is an Enzyme Immunoassay kit for the quantification of Mouse CD127 / IL7R in serum and cell culture supernatants. |
|---|---|
| 反应物种 | Ms |
| 应用 | ELISA |
| 特异性 | There is no detectable cross-reactivity with other relevant proteins. |
| 靶点名称 | CD127 / IL7R |
| 偶联标记 | HRP |
| 偶联标记说明 | Substrate: TMB and read at 450 nm. |
| 灵敏度 | 15.6 pg/ml |
| 样品类型 | Serum and cell culture supernatants. |
| 标准范围 | 31.2 - 2000 pg/ml |
| 样本量 | 100 µl |
| 精确 | Intra-Assay CV: 5.6%; Inter-Assay CV: 6.7% |
| 別名 | ILRA; CD127; IL-7R subunit alpha; IL-7R-alpha; IL7RA; CD antigen CD127; Interleukin-7 receptor subunit alpha; CDW127; CDw127; IL-7 receptor subunit alpha; IL-7RA |
应用说明
| 检测时间 | ~ 5 hours |
|---|
属性
| 形式 | 96 well |
|---|---|
| 存放说明 | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | Swiss-port # P16872 Mouse Interleukin-7 receptor subunit alpha |
|---|---|
| 基因名称 | IL7R |
| 全名 | interleukin 7 receptor |
| 背景介绍 | The protein encoded by this gene is a receptor for interleukine 7 (IL7). The function of this receptor requires the interleukin 2 receptor, gamma chain (IL2RG), which is a common gamma chain shared by the receptors of various cytokines, including interleukine 2, 4, 7, 9, and 15. This protein has been shown to play a critical role in the V(D)J recombination during lymphocyte development. This protein is also found to control the accessibility of the TCR gamma locus by STAT5 and histone acetylation. Knockout studies in mice suggested that blocking apoptosis is an essential function of this protein during differentiation and activation of T lymphocytes. The functional defects in this protein may be associated with the pathogenesis of the severe combined immunodeficiency (SCID). Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, May 2014] |
| 生物功能 | Receptor for interleukin-7. Also acts as a receptor for thymic stromal lymphopoietin (TSLP). [UniProt] |
| 产品亮点 | Related products: CD127 antibodies; CD127 ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| 翻译后修饰 | N-glycosylated IL-7Ralpha binds IL7 300-fold more tightly than the unglycosylated form. [UniProt] |
检测图片 (1)
ARG81723 Mouse CD127 / IL7R ELISA Kit standard curve image
ARG81723 Mouse CD127 / IL7R ELISA Kit results of a typical standard run with optical density reading at 450 nm.
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