Mouse CD153 / CD30 Ligand ELISA Kit
Key features and details
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- 偶联标记说明:
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Brand:
CAT.NO. : ARG82691
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Product Details
内含物
| 货号 | 内含物名称 | 包装 | 温度 |
|---|---|---|---|
| ARG82691-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG82691-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG82691-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG82691-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82691-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82691-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82691-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82691-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG82691-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG82691-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG82691-011 | Plate sealer | 4 strips | Room temperature |
概述
| 产品描述 | ARG82691 Mouse CD153 / CD30 Ligand ELISA Kit is an Enzyme Immunoassay kit for the quantification of Mouse CD153 / CD30 Ligand in serum, plasma (EDTA, heparin) and cell culture supernatants. |
|---|---|
| 反应物种 | Ms |
| 应用 | ELISA |
| 靶点名称 | CD153 / CD30 Ligand |
| 偶联标记 | HRP |
| 偶联标记说明 | Substrate: TMB and read at 450 nm. |
| 灵敏度 | 8 pg/ml |
| 样品类型 | Serum, plasma (EDTA, heparin) and cell culture supernatants. |
| 标准范围 | 15.6 - 1000 pg/ml |
| 样本量 | 100 µl |
| 精确 | Intra-Assay CV: 6.9% Inter-Assay CV: 7.9% |
| 別名 | Tumor necrosis factor ligand superfamily member 8; CD30L; CD30-L; CD153; CD antigen CD153; CD30LG; CD30 ligand |
应用说明
| 检测时间 | ~ 5 hours |
|---|
属性
| 形式 | 96 well |
|---|---|
| 存放说明 | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | Swiss-port # P32972 Mouse Tumor necrosis factor ligand superfamily member 8 |
|---|---|
| 基因名称 | TNFSF8 |
| 全名 | tumor necrosis factor (ligand) superfamily, member 8 |
| 背景介绍 | The protein encoded by this gene is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This cytokine is a ligand for TNFRSF8/CD30, which is a cell surface antigen and a marker for Hodgkin lymphoma and related hematologic malignancies. The engagement of this cytokine expressed on B cell surface plays an inhibitory role in modulating Ig class switch. This cytokine was shown to enhance cell proliferation of some lymphoma cell lines, while to induce cell death and reduce cell proliferation of other lymphoma cell lines. The pleiotropic biologic activities of this cytokine on different CD30+ lymphoma cell lines may play a pathophysiologic role in Hodgkin's and some non-Hodgkin's lymphomas. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2011] |
| 生物功能 | Cytokine that binds to TNFRSF8/CD30. Induces proliferation of T-cells. [UniProt] |
| 细胞定位 | Membrane; Single-pass type II membrane protein. [UniProt] |
| 产品亮点 | Related products: CD153 antibodies; CD153 ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
检测图片 (1)
ARG82691 Mouse CD153 / CD30 Ligand ELISA Kit standard curve image
ARG82691 Mouse CD153 / CD30 Ligand ELISA Kit results of a typical standard run with optical density reading at 450 nm.
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