Mouse CD25 ELISA Kit
Key features and details
- 产品描述:
- 反应物种:
- 应用:
- 特异性:
- 靶点名称:
- 偶联标记:
- 偶联标记说明:
- 灵敏度:
- 样品类型:
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Brand:
CAT.NO. : ARG81776
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Product Details
内含物
| 货号 | 内含物名称 | 包装 | 温度 |
|---|---|---|---|
| ARG81776-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81776-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG81776-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81776-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81776-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81776-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81776-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81776-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81776-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81776-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81776-011 | Plate sealer | 4 strips | Room temperature |
概述
| 产品描述 | ARG81776 Mouse CD25 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Mouse CD25 in serum, plasma (heparin, EDTA) and cell culture supernatants. |
|---|---|
| 反应物种 | Ms |
| 应用 | ELISA |
| 特异性 | There is no detectable cross-reactivity with other relevant proteins. |
| 靶点名称 | CD25 |
| 偶联标记 | HRP |
| 偶联标记说明 | Substrate: TMB and read at 450 nm. |
| 灵敏度 | 39 pg/ml |
| 样品类型 | Serum, plasma (heparin, EDTA) and cell culture supernatants. |
| 标准范围 | 78 - 5000 pg/ml |
| 样本量 | 100 µl |
| 精确 | Intra-Assay CV: 5.9%; Inter-Assay CV: 7.0% |
| 別名 | IL-2-RA; IL-2 receptor subunit alpha; CD25; TCGFR; TAC antigen; IL2R; CD antigen CD25; Interleukin-2 receptor subunit alpha; IL-2R subunit alpha; p55; IL2-RA; IDDM10 |
应用说明
| 检测时间 | ~ 5 hours |
|---|
属性
| 形式 | 96 well |
|---|---|
| 存放说明 | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | Swiss-port # P01590 Mouse Interleukin-2 receptor subunit alpha |
|---|---|
| 基因名称 | IL2RA |
| 全名 | interleukin 2 receptor, alpha |
| 背景介绍 | The interleukin 2 (IL2) receptor alpha (IL2RA) and beta (IL2RB) chains, together with the common gamma chain (IL2RG), constitute the high-affinity IL2 receptor. Homodimeric alpha chains (IL2RA) result in low-affinity receptor, while homodimeric beta (IL2RB) chains produce a medium-affinity receptor. Normally an integral-membrane protein, soluble IL2RA has been isolated and determined to result from extracellular proteolyisis. Alternately-spliced IL2RA mRNAs have been isolated, but the significance of each is presently unknown. Mutations in this gene are associated with interleukin 2 receptor alpha deficiency.[provided by RefSeq, Nov 2009] |
| 生物功能 | Receptor for interleukin-2. [UniProt] |
| 产品亮点 | Related products: CD25 antibodies; CD25 ELISA Kits; CD25 recombinant proteins; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
检测图片 (1)
ARG81776 Mouse CD25 ELISA Kit standard curve image
ARG81776 Mouse CD25 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
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