Mouse CD40 ELISA Kit
Key features and details
- 产品描述:
- 反应物种:
- 应用:
- 特异性:
- 靶点名称:
- 偶联标记:
- 偶联标记说明:
- 灵敏度:
- 样品类型:
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Brand:
CAT.NO. : ARG81717
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Product Details
内含物
| 货号 | 内含物名称 | 包装 | 温度 |
|---|---|---|---|
| ARG81717-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81717-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG81717-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81717-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81717-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81717-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81717-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81717-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81717-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81717-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81717-011 | Plate sealer | 4 strips | Room temperature |
概述
| 产品描述 | ARG81717 Mouse CD40 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Mouse CD40 in serum, plasma (heparin, EDTA) and cell culture supernatants. |
|---|---|
| 反应物种 | Ms |
| 应用 | ELISA |
| 特异性 | There is no detectable cross-reactivity with other relevant proteins. |
| 靶点名称 | CD40 |
| 偶联标记 | HRP |
| 偶联标记说明 | Substrate: TMB and read at 450 nm. |
| 灵敏度 | 7.8 pg/ml |
| 样品类型 | Serum, plasma (heparin, EDTA) and cell culture supernatants. |
| 标准范围 | 15.6 - 1000 pg/ml |
| 样本量 | 100 µl |
| 精确 | Intra-Assay CV: 5.2%; Inter-Assay CV: 6.3% |
| 別名 | CDw40; CD antigen CD40; Tumor necrosis factor receptor superfamily member 5; Bp50; CD40L receptor; CDW40; TNFRSF5; p50; B-cell surface antigen CD40 |
应用说明
| 检测时间 | ~ 5 hours |
|---|
属性
| 形式 | 96 well |
|---|---|
| 存放说明 | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | Swiss-port # P27512 Mouse Tumor necrosis factor receptor superfamily member 5 |
|---|---|
| 基因名称 | CD40 |
| 全名 | CD40 molecule, TNF receptor superfamily member 5 |
| 背景介绍 | This gene is a member of the TNF-receptor superfamily. The encoded protein is a receptor on antigen-presenting cells of the immune system and is essential for mediating a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation. AT-hook transcription factor AKNA is reported to coordinately regulate the expression of this receptor and its ligand, which may be important for homotypic cell interactions. Adaptor protein TNFR2 interacts with this receptor and serves as a mediator of the signal transduction. The interaction of this receptor and its ligand is found to be necessary for amyloid-beta-induced microglial activation, and thus is thought to be an early event in Alzheimer disease pathogenesis. Mutations affecting this gene are the cause of autosomal recessive hyper-IgM immunodeficiency type 3 (HIGM3). Multiple alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq, Nov 2014] |
| 生物功能 | Receptor for TNFSF5/CD40LG. Transduces TRAF6- and MAP3K8-mediated signals that activate ERK in macrophages and B cells, leading to induction of immunoglobulin secretion. [UniProt] |
| 产品亮点 | Related products: CD40 antibodies; CD40 ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
检测图片 (1)
ARG81717 Mouse CD40 ELISA Kit standard curve image
ARG81717 Mouse CD40 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
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