Mouse CD95 / Fas ELISA Kit
Key features and details
- 产品描述:
- 反应物种:
- 应用:
- 特异性:
- 靶点名称:
- 偶联标记:
- 偶联标记说明:
- 灵敏度:
- 样品类型:
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Brand:
CAT.NO. : ARG81642
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Product Details
内含物
| 货号 | 内含物名称 | 包装 | 温度 |
|---|---|---|---|
| ARG81642-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81642-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG81642-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81642-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81642-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81642-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81642-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81642-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81642-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81642-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81642-011 | Plate sealer | 4 strips | Room temperature |
概述
| 产品描述 | ARG81642 Mouse CD95 / Fas ELISA Kit is an Enzyme Immunoassay kit for the quantification of Mouse CD95 / Fas in serum, plasma (heparin, EDTA, citrate) and cell culture supernatants. |
|---|---|
| 反应物种 | Ms |
| 应用 | ELISA |
| 特异性 | There is no detectable cross-reactivity with other relevant proteins. |
| 靶点名称 | CD95 / Fas |
| 偶联标记 | HRP |
| 偶联标记说明 | Substrate: TMB and read at 450 nm. |
| 灵敏度 | 15.6 pg/ml |
| 样品类型 | Serum, plasma (heparin, EDTA, citrate) and cell culture supernatants. |
| 标准范围 | 31.2 - 2000 pg/ml |
| 样本量 | 100 µl |
| 精确 | Intra-Assay CV: 6.2%; Inter-Assay CV: 7.3% |
| 別名 | CD95; Apoptosis-mediating surface antigen FAS; FAS1; Tumor necrosis factor receptor superfamily member 6; ALPS1A; APT1; FASTM; CD antigen CD95; APO-1; TNFRSF6; FASLG receptor; Apo-1 antigen |
应用说明
| 检测时间 | ~ 5 hours |
|---|
属性
| 形式 | 96 well |
|---|---|
| 存放说明 | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | Swiss-port # P25446 Mouse Tumor necrosis factor receptor superfamily member 6 |
|---|---|
| 基因名称 | FAS |
| 全名 | Fas cell surface death receptor |
| 背景介绍 | The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor contains a death domain. It has been shown to play a central role in the physiological regulation of programmed cell death, and has been implicated in the pathogenesis of various malignancies and diseases of the immune system. The interaction of this receptor with its ligand allows the formation of a death-inducing signaling complex that includes Fas-associated death domain protein (FADD), caspase 8, and caspase 10. The autoproteolytic processing of the caspases in the complex triggers a downstream caspase cascade, and leads to apoptosis. This receptor has been also shown to activate NF-kappaB, MAPK3/ERK1, and MAPK8/JNK, and is found to be involved in transducing the proliferating signals in normal diploid fibroblast and T cells. Several alternatively spliced transcript variants have been described, some of which are candidates for nonsense-mediated mRNA decay (NMD). The isoforms lacking the transmembrane domain may negatively regulate the apoptosis mediated by the full length isoform. [provided by RefSeq, Mar 2011] |
| 生物功能 | Receptor for TNFSF6/FASLG. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. The secreted isoforms 2 to 6 block apoptosis (in vitro). [UniProt] |
| 产品亮点 | Related products: CD95 antibodies; CD95 ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| 翻译后修饰 | N- and O-glycosylated. O-glycosylated with core 1 or possibly core 8 glycans. [UniProt] |
检测图片 (1)
ARG81642 Mouse CD95 / Fas ELISA Kit standard curve image
ARG81642 Mouse CD95 / Fas ELISA Kit results of a typical standard run with optical density reading at 450 nm.
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