Mouse Coagulation Factor XII ELISA Kit
Protocol Booklet
Key features and details
- Specification:
- Sensitivity:
- Standard Curve Range:
- Standard Curve Gradient:
- Number of Incubations:
- Sample Volume:
- Assay type:
- Operation Duration:
-
Brand:
Product Details
|
pg/ml |
O.D. |
Average |
Corrected |
|
|
0.00 |
0.0164 |
0.0142 |
0.0153 |
|
|
13.72 |
0.0253 |
0.0224 |
0.0239 |
0.0086 |
|
41.15 |
0.0286 |
0.0276 |
0.0281 |
0.0128 |
|
123.46 |
0.0472 |
0.0489 |
0.0481 |
0.0328 |
|
370.37 |
0.1131 |
0.1289 |
0.1210 |
0.1057 |
|
1111.11 |
0.3938 |
0.4223 |
0.4081 |
0.3928 |
|
3333.33 |
1.3840 |
1.4040 |
1.3940 |
1.3787 |
|
10000.00 |
3.3980 |
3.3740 |
3.3860 |
3.3707 |
|
Intra-assay Precision |
|
Inter-assay Precision |
|||||
|
Sample Number |
S1 |
S2 |
S3 |
S1 |
S2 |
S3 |
|
|
22 |
22 |
22 |
6 |
6 |
6 |
||
|
Average（pg/ml） |
186.2 |
1021.9 |
3453.9 |
143.3 |
846.7 |
2856.7 |
|
|
Standard deviation |
5.7 |
43.2 |
230.7 |
6.7 |
56.7 |
113.3 |
|
|
Coefficient of variation（%） |
3.0 |
4.2 |
6.7 |
5.5 |
6.6 |
4.0 |
|
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
The spike recovery was evaluated by spiking 3 levels of Mouse Coagulation Factor XII into health Mouse serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 93% to 106% with an overall mean recovery of 100%.
|
Sample Matrix |
Sample Evaluated |
Range (ug/ml) |
Detectable % |
Mean of Detectable (ug/ml) |
|
Serum |
30 |
13.00-60.31 |
100 |
33.76 |
Serum/Plasma – Thirty samples from apparently healthy mice were evaluated for the presence of Coagulation Factor XII in this assay. No medical histories were available for the donors. n.d. = non-detectable. Samples measured below the sensitivity are considered to be non-detectable.
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