Mouse Coagulation Factor XII ELISA Kit
Protocol Booklet
Key features and details
- Specification:
- Sensitivity:
- Standard Curve Range:
- Standard Curve Gradient:
- Number of Incubations:
- Sample Volume:
- Assay type:
- Operation Duration:
-
Brand:
Product Details
|
ng/ml |
O.D. |
Average |
Corrected |
|
|
0.00 |
0.0060 |
0.0054 |
0.0057 |
|
|
4.69 |
0.0766 |
0.0693 |
0.0730 |
0.0673 |
|
9.38 |
0.1558 |
0.1445 |
0.1502 |
0.1445 |
|
18.75 |
0.2989 |
0.3013 |
0.3001 |
0.2944 |
|
37.50 |
0.6302 |
0.5610 |
0.5956 |
0.5899 |
|
75.00 |
1.2021 |
1.2191 |
1.2106 |
1.2049 |
|
150.00 |
2.4639 |
2.3883 |
2.4261 |
2.4204 |
|
300.00 |
4.0653 |
4.0355 |
4.0504 |
4.0447 |
|
Intra-assay Precision |
|
Inter-assay Precision |
|||||
|
Sample Number |
S1 |
S2 |
S3 |
S1 |
S2 |
S3 |
|
|
22 |
22 |
22 |
6 |
6 |
6 |
||
|
Average（ng/ml） |
5.6 |
30.7 |
103.6 |
4.3 |
25.4 |
85.7 |
|
|
Standard deviation |
0.2 |
1.3 |
6.9 |
0.2 |
1.7 |
3.4 |
|
|
Coefficient of variation（%） |
3.0 |
4.2 |
6.7 |
5.5 |
6.6 |
4.0 |
|
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
The recovery ranged from 84% to 122% with an overall mean recovery of 105%.
|
Sample Matrix |
Sample Evaluated |
Range (ug/ml) |
Detectable % |
Mean of Detectable (ug/ml) |
|
Serum |
30 |
29.16-54.27 |
100.0% |
42.19 |
New Products
