Mouse CPE / Carboxypeptidase E ELISA Kit
Key features and details
- 产品描述:
- 反应物种:
- 应用:
- 靶点名称:
- 偶联标记:
- 偶联标记说明:
- 灵敏度:
- 样品类型:
- 标准范围:
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Brand:
CAT.NO. : ARG82413
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Product Details
内含物
| 货号 | 内含物名称 | 包装 | 温度 |
|---|---|---|---|
| ARG82413-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG82413-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG82413-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG82413-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82413-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82413-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82413-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82413-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG82413-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG82413-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG82413-011 | Plate sealer | 4 strips | Room temperature |
概述
| 产品描述 | ARG82413 Mouse CPE / Carboxypeptidase E ELISA Kit is an Enzyme Immunoassay kit for the quantification of Mouse CPE / Carboxypeptidase E in serum, plasma (EDTA, heparin) and cell culture supernatants. |
|---|---|
| 反应物种 | Ms |
| 应用 | ELISA |
| 靶点名称 | CPE / Carboxypeptidase E |
| 偶联标记 | HRP |
| 偶联标记说明 | Substrate: TMB and read at 450 nm. |
| 灵敏度 | 78 pg/ml |
| 样品类型 | Serum, plasma (EDTA, heparin) and cell culture supernatants. |
| 标准范围 | 156 - 10000 pg/ml |
| 样本量 | 100 µl |
| 精确 | Intra-Assay CV: 6.0% Inter-Assay CV: 6.6% |
| 別名 | CPH; Carboxypeptidase H; Enkephalin convertase; CPE; Carboxypeptidase E; Prohormone-processing carboxypeptidase; EC 3.4.17.10 |
应用说明
| 检测时间 | ~ 5 hours |
|---|
属性
| 形式 | 96 well |
|---|---|
| 存放说明 | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | CPE |
| 全名 | carboxypeptidase E |
| 背景介绍 | This gene encodes a carboxypeptidase that cleaves C-terminal amino acid residues and is involved in the biosynthesis of peptide hormones and neurotransmitters, including insulin. It is a peripheral membrane protein. The protein specifically binds regulated secretory pathway proteins, including prohormones, but not constitutively secreted proteins. Mutations in this gene are implicated in type II diabetes. [provided by RefSeq, Jul 2008] |
| 生物功能 | Removes residual C-terminal Arg or Lys remaining after initial endoprotease cleavage during prohormone processing. Processes proinsulin. [UniProt] |
| 细胞定位 | Isoform 1: Cytoplasmic vesicle, secretory vesicle membrane; Peripheral membrane protein. Secreted. Note=Associated with the secretory granule membrane through direct binding to lipid rafts in intragranular conditions. Isoform 2: Nucleus. [UniProt] |
| 产品亮点 | Related products: Carboxypeptidase antibodies; Carboxypeptidase ELISA Kits; Carboxypeptidase recombinant proteins; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
检测图片 (1)
ARG82413 Mouse CPE / Carboxypeptidase E ELISA Kit standard curve image
ARG82413 Mouse CPE / Carboxypeptidase E ELISA Kit results of a typical standard run with optical density reading at 450 nm.
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