Mouse CXADR / CAR ELISA Kit
Key features and details
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- 反应物种:
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- 靶点名称:
- 偶联标记:
- 偶联标记说明:
- 灵敏度:
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Brand:
CAT.NO. : ARG82378
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Product Details
内含物
| 货号 | 内含物名称 | 包装 | 温度 |
|---|---|---|---|
| ARG82378-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG82378-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG82378-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG82378-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82378-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82378-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82378-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82378-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG82378-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG82378-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG82378-011 | Plate sealer | 4 strips | Room temperature |
概述
| 产品描述 | ARG82378 Mouse CXADR / CAR ELISA Kit is an Enzyme Immunoassay kit for the quantification of Mouse CXADR / CAR in serum, plasma (EDTA, heparin, citrate) and cell culture supernatants. |
|---|---|
| 反应物种 | Ms |
| 应用 | ELISA |
| 靶点名称 | CXADR / CAR |
| 偶联标记 | HRP |
| 偶联标记说明 | Substrate: TMB and read at 450 nm. |
| 灵敏度 | 15.6 pg/ml |
| 样品类型 | Serum, plasma (EDTA, heparin, citrate) and cell culture supernatants. |
| 标准范围 | 31.2 - 2000 pg/ml |
| 样本量 | 100 µl |
| 精确 | Intra-Assay CV: 5.5% Inter-Assay CV: 6.2% |
| 別名 | Coxsackievirus B-adenovirus receptor; CAR4/6; HCAR; hCAR; CAR; Coxsackievirus and adenovirus receptor; CVB3-binding protein; HCVADR |
应用说明
| 检测时间 | ~ 5 hours |
|---|
属性
| 形式 | 96 well |
|---|---|
| 存放说明 | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | Swiss-port # P97792 Mouse Coxsackievirus and adenovirus receptor homolog |
|---|---|
| 基因名称 | CXADR |
| 全名 | coxsackie virus and adenovirus receptor |
| 背景介绍 | The protein encoded by this gene is a type I membrane receptor for group B coxsackieviruses and subgroup C adenoviruses. Several transcript variants encoding different isoforms have been found for this gene. Pseudogenes of this gene are found on chromosomes 15, 18, and 21. [provided by RefSeq, May 2011] |
| 生物功能 | Component of the epithelial apical junction complex that may function as an homophilic cell adhesion molecule and is essential for tight junction integrity. Also involved in transepithelial migration of leukocytes through adhesive interactions with AMICA1/JAML a transmembrane protein of the plasma membrane of leukocytes. The interaction between both receptors also mediates the activation of gamma-delta T-cells, a subpopulation of T-cells residing in epithelia and involved in tissue homeostasis and repair. Upon epithelial CXADR-binding, AMICA1 induces downstream cell signaling events in gamma-delta T-cells through PI3-kinase and MAP kinases. It results in proliferation and production of cytokines and growth factors by T-cells that in turn stimulate epithelial tissues repair. [UniProt] |
| 细胞定位 | Isoform 1: Cell membrane; Single-pass type I membrane protein. Basolateral cell membrane; Single-pass type I membrane protein. Cell junction, tight junction. Cell junction, adherens junction. Note=In epithelial cells localizes to the apical junction complex composed of tight and adherens junctions (PubMed:12297051). In airway epithelial cells localized to basolateral membrane but not to apical surface (PubMed:11316797). Isoform 3: Secreted. Isoform 4: Secreted. Isoform 5: Secreted. [UniProt] |
| 产品亮点 | Related products: CXADR antibodies; CXADR ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| 翻译后修饰 | N-glycosylated. Palmitoylated on Cys-259 and/or Cys-260; required for proper localization to the plasma membrane. [UniProt] |
检测图片 (1)
ARG82378 Mouse CXADR / CAR ELISA Kit standard curve image
ARG82378 Mouse CXADR / CAR ELISA Kit results of a typical standard run with optical density reading at 450 nm.
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