Mouse CXCL1/KC ELISA Kit
Protocol Booklet
Key features and details
- Specification:
- Sensitivity:
- Standard Curve Range:
- Standard Curve Gradient:
- Number of Incubations:
- Sample Volume:
- Assay type:
- Operation Duration:
-
Brand:
CAT.NO. : AEM0017
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Size:
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Product Details
Background
CXCL1/GRO alpha, also known as KC in mouse and CINC-1 in rat, is a member of the CXC family of chemokines. Human CXCL1/GRO alpha is 107 amino acids (aa) in length with a predicted molecular weight of 11 kDa. The mouse and rat orthologs share 57% and 59% amino acid sequence identity with the human protein, respectively. Genome-wide analysis suggests that the protein encoded by the mouse Cxcl3 gene is homologous to human CXCL1/GRO alpha protein. Human CXCL1/GRO alpha can be cleaved into three isoforms CXCL1/GRO alpha (aa 4-73), CXCL1/GRO alpha (aa 5-73), and CXCL1/GRO alpha (aa 6-73) which show higher activity than the full length protein. CXCL1/GRO alpha is secreted by mast cells and macrophages whereupon it acts as a chemoattractant for neutrophils.
Typical data
| pg/ml | O.D. | Average | Corrected | |
| 0.00 | 0.0104 | 0.0085 | 0.0095 | |
| 13.72 | 0.0192 | 0.0193 | 0.0193 | 0.0098 |
| 41.15 | 0.0391 | 0.0402 | 0.0397 | 0.0302 |
| 123.46 | 0.0841 | 0.0835 | 0.0838 | 0.0743 |
| 370.37 | 0.2060 | 0.2181 | 0.2121 | 0.2026 |
| 1111.11 | 0.6431 | 0.6263 | 0.6347 | 0.6252 |
| 3333.33 | 1.6330 | 1.8620 | 1.7475 | 1.7380 |
| 10000.00 | 4.0881 | 4.1300 | 4.1091 | 4.0996 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average(pg/ml) | 217.4 | 917.4 | 3162 | 158.9 | 706.9 | 2846.8 |
| Standard Deviation | 14.6 | 51.4 | 187.2 | 9.2 | 29.9 | 172.5 |
| Coefficient of Variation(%) | 6.7 | 5.6 | 5.9 | 5.8 | 4.2 | 6.1 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of mouse CXCL1/KC into health mouse serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 88% to 109% with an overall mean recovery of 96%.
The recovery ranged from 88% to 109% with an overall mean recovery of 96%.
Sample Values
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
| Serum | 30 | 43.94-268.81 | 100 | 66.43 |
Serum/Plasma – Thirty samples from apparently healthy mice were evaluated for the presence of CXCL1/KC in this assay. No medical histories were available for the donors. n.d. = non-detectable. Samples measured below the sensitivity are considered to be non-detectable.
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