Mouse CXCL10/IP-10/CRG-2 ELISA Kit
Protocol Booklet
Key features and details
- Specification:
- Sensitivity:
- Standard Curve Range:
- Standard Curve Gradient:
- Number of Incubations:
- Sample Volume:
- Assay type:
- Operation Duration:
-
Brand:
CAT.NO. : AEM0014
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Size:
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Product Details
Background
IP-10 was originally identified as an IFN-gamma-inducible gene in monocytes, fibroblasts and endothelial cells. The mouse homolog of human IP-10, CRG-2, shares approximately 67% amino acid sequence identity with human IP-10. The amino acid sequence of IP-10 identified the protein as a member of the CXC chemokine subfamily.
Typical data
| pg/ml | O.D. | Average | Corrected | |
| 0.00 | 0.0160 | 0.0160 | 0.0160 | |
| 312.50 | 0.0190 | 0.0210 | 0.0200 | 0.0040 |
| 625.00 | 0.0290 | 0.0270 | 0.0280 | 0.0120 |
| 1250.00 | 0.0500 | 0.0490 | 0.0495 | 0.0335 |
| 2500.00 | 0.1120 | 0.1110 | 0.1115 | 0.0955 |
| 5000.00 | 0.3180 | 0.3180 | 0.3180 | 0.3020 |
| 10000.00 | 0.8800 | 0.7990 | 0.8395 | 0.8235 |
| 20000.00 | 2.0520 | 1.9860 | 2.0190 | 2.0030 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average(pg/ml) | 1023.2 | 3998.7 | 8588.8 | 1179.3 | 4202.1 | 8465.1 |
| Standard Deviation | 62.1 | 188.3 | 664.3 | 79 | 111.8 | 378.3 |
| Coefficient of Variation(%) | 6.1 | 4.7 | 7.7 | 6.7 | 2.7 | 4.5 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of mouse CXCL10 into health mouse serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 86% to 114% with an overall mean recovery of 97%.
The recovery ranged from 86% to 114% with an overall mean recovery of 97%.
Sample Values
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
| Serum | 30 | n.d.-437.15 | 21 | 279.03 |
Serum/Plasma – Thirty samples from apparently healthy mice were evaluated for the presence of CXCL10 in this assay. No medical histories were available for the donors. n.d. = non-detectable. Samples measured below the sensitivity are considered to be non-detectable.
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