Mouse CXCL2 ELISA Kit
Protocol Booklet
Key features and details
- Specification:
- Sensitivity:
- Standard Curve Range:
- Standard Curve Gradient:
- Number of Incubations:
- Sample Volume:
- Assay type:
- Operation Duration:
-
Brand:
CAT.NO. : AEM0018
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Size:
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Product Details
Background
CXCL2/GRO beta, also called MIP-2 in mouse and CINC-3 in rat, is a member of the CXC chemokine family. Human CXCL2/GRO beta is 107 amino acids (aa) in length with a predicted molecular weight of 11 kDa. The mouse and rat orthologs share 70% and 71% aa sequence identity with the human protein, respectively. N-terminal aa 1-4 of CXCL2/GRO beta can be post-translationally cleaved which confers enhanced hematopoietic bioactivity. CXCL2/GRO beta is produced by a variety of cell types including monocytes and macrophages at sites of inflammation and is chemotactic for granulocytes, including neutrophils.
Typical data
| pg/ml | O.D. | Average | Corrected | |
| 0.00 | 0.0130 | 0.0122 | 0.0126 | |
| 13.72 | 0.0151 | 0.0142 | 0.0147 | 0.0021 |
| 41.15 | 0.0210 | 0.0210 | 0.0210 | 0.0084 |
| 123.46 | 0.0381 | 0.0411 | 0.0396 | 0.0270 |
| 370.37 | 0.1251 | 0.1312 | 0.1282 | 0.1156 |
| 1111.11 | 0.5023 | 0.5012 | 0.5018 | 0.4892 |
| 3333.33 | 1.7271 | 1.8113 | 1.7692 | 1.7566 |
| 10000.00 | 3.9910 | 4.0931 | 4.0421 | 4.0295 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average(pg/ml) | 160.2 | 1021.4 | 3095.3 | 153.9 | 1034.9 | 3264.4 |
| Standard Deviation | 8.9 | 64.2 | 148.8 | 9.0 | 55.2 | 180.8 |
| Coefficient of Variation(%) | 5.6 | 6.3 | 4.8 | 5.9 | 5.3 | 5.5 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of mouse CXCL2/MIP-2 into health mouse serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 89% to 114% with an overall mean recovery of 101%.
The recovery ranged from 89% to 114% with an overall mean recovery of 101%.
Sample Values
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
| Serum | 30 | n.d. | 0 | n.d. |
Serum/Plasma – Thirty samples from apparently healthy mice were evaluated for the presence of CXCL2/MIP-2 in this assay. No medical histories were available for the donors. n.d. = non-detectable. Samples measured below the sensitivity are considered to be non-detectable.
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