Mouse GM-CSF/CSF2 ELISA Kit
Protocol Booklet
Key features and details
- Specification:
- Sensitivity:
- Standard Curve Range:
- Standard Curve Gradient:
- Number of Incubations:
- Sample Volume:
- Assay type:
- Operation Duration:
-
Brand:
CAT.NO. : AEM0011
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Size:
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Product Details
Background
GM-CSF (Granulocyte-Macrophage Colony Stimulating Factor) induces the development of monocytes, neutrophils, eosinophils, and myeloid and dermal dendritic cells. It also acts as a neutrophil and dendritic cell chemoattractant. GM-CSF promotes Th1 and Th17 cell mediated autoimmune inflammation as well as the inflammatory activation of dendritic cells, microglia, alveolar macrophages, and eosinophils. In addition, it cooperates with G-CSF in promoting tumor cell proliferation and invasion. GM-CSF signals through a receptor complex composed of GM-CSF R alpha and the Common beta Chain (beta c) with Syndecan-2 as a potential co-receptor. The beta c subunit is shared by the receptor complexes for IL-3 and IL-5.
Typical data
| pg/ml | O.D. | Average | Corrected | |
| 0.00 | 0.0831 | 0.0801 | 0.0816 | |
| 0.69 | 0.1011 | 0.0991 | 0.1001 | 0.0185 |
| 2.06 | 0.1453 | 0.1384 | 0.1419 | 0.0603 |
| 6.17 | 0.2796 | 0.2752 | 0.2774 | 0.1958 |
| 18.52 | 0.6674 | 0.5825 | 0.6250 | 0.5434 |
| 55.56 | 1.5812 | 1.4493 | 1.5153 | 1.4337 |
| 166.67 | 3.4412 | 3.1762 | 3.3087 | 3.2271 |
| 500.00 | 4.3492 | 4.3873 | 4.3683 | 4.2867 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average(pg/ml) | 7.6 | 29.4 | 162.5 | 8.2 | 32.2 | 145.1 |
| Standard Deviation | 0.5 | 1.5 | 9.8 | 0.6 | 2.0 | 7.3 |
| Coefficient of Variation(%) | 6.0 | 5.1 | 6.0 | 6.9 | 6.3 | 5.0 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of mouse GM-CSF/CSF2 into health mouse serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 81% to 103% with an overall mean recovery of 92%.
The recovery ranged from 81% to 103% with an overall mean recovery of 92%.
Sample Values
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
| Serum | 30 | n.d | 0 | 0 |
Serum/Plasma – Thirty samples from apparently healthy mice were evaluated for the presence of GM-CSF/CSF2 in this assay. No medical histories were available for the donors. n.d. = non-detectable. Samples measured below the sensitivity are considered to be non-detectable.
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