Mouse IFNAR1 ELISA Kit
Key features and details
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- 反应物种:
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- 靶点名称:
- 偶联标记:
- 偶联标记说明:
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- 标准范围:
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Brand:
CAT.NO. : ARG82459
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Product Details
内含物
| 货号 | 内含物名称 | 包装 | 温度 |
|---|---|---|---|
| ARG82459-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG82459-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG82459-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG82459-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82459-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82459-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82459-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82459-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG82459-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG82459-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG82459-011 | Plate sealer | 4 strips | Room temperature |
概述
| 产品描述 | ARG82459 Mouse IFNAR1 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Mouse IFNAR1 in serum, plasma (EDTA, heparin) and cell culture supernatants. |
|---|---|
| 反应物种 | Ms |
| 应用 | ELISA |
| 靶点名称 | IFNAR1 |
| 偶联标记 | HRP |
| 偶联标记说明 | Substrate: TMB and read at 450 nm. |
| 灵敏度 | 31.25 pg/ml |
| 样品类型 | Serum, plasma (EDTA, heparin) and cell culture supernatants. |
| 标准范围 | 62.5 - 4000 pg/ml |
| 样本量 | 100 µl |
| 精确 | Intra-Assay CV: 4.3% Inter-Assay CV: 5.8% |
| 別名 | Cytokine receptor class-II member 1; Cytokine receptor family 2 member 1; IFRC; IFNAR; AVP; IFN-alpha/beta receptor 1; CRF2-1; IFN-R-1; Type I interferon receptor 1; IFNBR; Interferon alpha/beta receptor 1; IFN-alpha-REC |
应用说明
| 检测时间 | ~ 5 hours |
|---|
属性
| 形式 | 96 well |
|---|---|
| 存放说明 | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | IFNAR1 |
| 全名 | interferon (alpha, beta and omega) receptor 1 |
| 背景介绍 | The protein encoded by this gene is a type I membrane protein that forms one of the two chains of a receptor for interferons alpha and beta. Binding and activation of the receptor stimulates Janus protein kinases, which in turn phosphorylate several proteins, including STAT1 and STAT2. The encoded protein also functions as an antiviral factor. [provided by RefSeq, Jul 2008] |
| 生物功能 | Associates with IFNAR2 to form the type I interferon receptor. Receptor for interferons alpha and beta. Binding to type I IFNs triggers tyrosine phosphorylation of a number of proteins including JAKs, TYK2, STAT proteins and IFNR alpha- and beta-subunits themselves. Can also transduce IFNB signals without the help of IFNAR2, and not activating the Jak-STAT pathway. [UniProt] |
| 细胞定位 | Isoform 1: Cell membrane; Single-pass type I membrane protein. Late endosome. Lysosome. Note=Interferon binding triggers internalization of the receptor from the cell membrane into endosomes and then into lysosomes. [UniProt] |
| 产品亮点 | Related products: IFNAR1 antibodies; IFNAR1 ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| 翻译后修饰 | Ubiquitinated, leading to its internalization and degradation (PubMed:14532120, PubMed:15337770). Polyubiquitinated via 'Lys-48'-linked and 'Lys-63'-linked ubiquitin chains, leading to receptor internalization and lysosomal degradation (PubMed:18056411). The 'Lys-63'-linked ubiquitin chains are cleaved off by the BRISC complex (PubMed:24075985). Phosphorylated on serine residues in response to interferon binding; this promotes interaction with FBXW11 and ubiquitination (PubMed:14532120, PubMed:15337770, PubMed:24075985). Phosphorylated on tyrosine residues by TYK2 tyrosine kinase (PubMed:7526154). Phosphorylated on tyrosine residues in response to interferon (PubMed:10049744). Palmitoylation at Cys-463 is required for the activation of STAT1 and STAT2. [UniProt] |
检测图片 (1)
ARG82459 Mouse IFNAR1 ELISA Kit standard curve image
ARG82459 Mouse IFNAR1 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
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