Mouse IgG (Total) ELISA Kit
Key features and details
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- 偶联标记:
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CAT.NO. : ARG80215
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Product Details
内含物
| 货号 | 内含物名称 | 包装 | 温度 |
|---|---|---|---|
| ARG80215-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG80215-002 | Standard (Lyophilized) | 3 X 10 ng/vial | 4°C |
| ARG80215-003 | Standard diluent buffer | 40 ml | 4°C |
| ARG80215-004 | Antibody conjugate concentrate | 400 µl | 4°C |
| ARG80215-005 | Antibody diluent buffer | 16 ml | 4°C |
| ARG80215-006 | HRP-Streptavidin concentrate | 400 µl | 4°C (Protect from light) |
| ARG80215-007 | HRP-Streptavidin diluent buffer | 16 ml | 4°C |
| ARG80215-008 | 20X Wash buffer | 50 ml | 4°C |
| ARG80215-009 | TMB substrate | 12ml | 4°C (Protect from light) |
| ARG80215-010 | STOP solution | 12ml | 4°C |
| ARG80215-011 | Plate sealer | 4 strips | Room temperature |
概述
| 产品描述 | ARG80215 Mouse IgG (Total) ELISA Kit is an Enzyme Immunoassay kit for the quantification of Mouse IgG (Total) in Serum, Plasma, Cell culture supernatants. |
|---|---|
| 反应物种 | Ms |
| 应用 | ELISA |
| 特异性 | This ELISA kit might cross-reactwithIgGfromother species. |
| 靶点名称 | IgG (Total) |
| 偶联标记 | HRP |
| 偶联标记说明 | Substrate: TMB and read at 450 nm |
| 灵敏度 | 0.08 ng/ml |
| 样品类型 | Serum, Plasma, Cell culture supernatants |
| 标准范围 | 0.156 - 10 ng/ml |
| 样本量 | 100 µl |
| 精确 | CV: less than10% |
| 別名 | Immunoglobulin G |
应用说明
| 检测时间 | 4 hours |
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属性
| 形式 | 96 well |
|---|---|
| 存放说明 | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 背景介绍 | Antibodies are major components of the immune system. IgG is the main antibody isotype found in blood and extracellular fluid allowing it to control infection of body tissues. By binding many kinds of pathogens—representing viruses, bacteria, and fungi—IgG protects the body from infection. It does this via several immune mechanisms: IgG-mediated binding of pathogens causes their immobilization and binding together via agglutination; IgG coating of pathogen surfaces (known as opsonization) allows their recognition and ingestion by phagocytic immune cells; IgG activates the classical pathway of the complement system, a cascade of immune protein production that results in pathogen elimination; IgG also binds and neutralizes toxins. IgG also plays an important role in antibody-dependent cell-mediated cytotoxicity (ADCC) and intracellular antibody-mediated proteolysis, in which it binds to TRIM21 (the receptor with greatest affinity to IgG in humans) in order to direct marked virions to the proteasome in the cytosol. IgG is also associated with Type II and Type III Hypersensitivity. IgG antibodies are generated following class switching and maturation of the antibody response and thus participate predominantly in the secondary immune response. IgG is secreted as a monomer that is small in size allowing it to easily perfuse tissues. It is the only isotype that has receptors to facilitate passage through the human placenta, thereby providing protection to the fetus in utero. Along with IgA secreted in the breast milk, residual IgG absorbed through the placenta provides the neonate with humoral immunity before its own immune system develops. Colostrum contains a high percentage of IgG, especially bovine colostrum. In individuals with prior immunity to a pathogen, IgG appears about 24–48 hours after antigenic stimulation. |
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| 产品亮点 | Related products: IgG antibodies; IgG ELISA Kits; IgG Duos / Panels; IgG recombinant proteins; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| 研究领域 | Immune System kit |
检测图片 (1)
ARG80215 Mouse IgG (Total) ELISA Kit standard curve image
ARG80215 Mouse IgG (Total) ELISA Kit results of a typical standard run with optical density reading at 450 nm.
参考文献
Follicular regulatory T cells are associated with β-cell autoimmunity and the development of type 1 diabetes.
ELISA / Mouse
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