Mouse IL-12p70 ELISA Kit
Protocol Booklet
Key features and details
- Specification:
- Sensitivity:
- Standard Curve Range:
- Standard Curve Gradient:
- Number of Incubations:
- Sample Volume:
- Assay type:
- Operation Duration:
-
Brand:
CAT.NO. : AEM0006
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Size:
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Product Details
Background
Interleukin 12 (IL-12), also known as natural killer cell stimulatory factor (NKSF) or cytotoxic lymphocyte maturation factor (CLMF), is a heterodimeric pleiotropic cytokine made up of a 40 kDa (p40) subunit and a 35 kDa (p35) subunit. The IL-12 p40 subunit is shared by IL-23, another heterodimeric cytokine that has biological activities similar to, as well as distinct from, IL-12. IL-12 is produced by macrophages and B cells and has been shown to have multiple effects on T cells and natural killer (NK) cells. While mouse IL-12 is active on both human and mouse cells, human IL-12 is not active on mouse cells.
Typical data
| pg/ml | O.D. | Average | Corrected | |
| 0.00 | 0.0200 | 0.0190 | 0.0195 | |
| 13.72 | 0.0251 | 0.0250 | 0.0251 | 0.0056 |
| 41.15 | 0.0410 | 0.0406 | 0.0408 | 0.0213 |
| 123.46 | 0.0830 | 0.0839 | 0.0835 | 0.0640 |
| 370.37 | 0.2035 | 0.2045 | 0.2040 | 0.1845 |
| 1111.11 | 0.5391 | 0.5450 | 0.5421 | 0.5226 |
| 3333.33 | 1.4430 | 1.4711 | 1.4571 | 1.4376 |
| 10000.00 | 3.3985 | 3.4496 | 3.4241 | 3.4046 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average(pg/ml) | 141.5 | 820 | 2943.1 | 163.9 | 969 | 2922.8 |
| Standard Deviation | 7.5 | 42 | 165 | 9.7 | 25.9 | 129.8 |
| Coefficient of Variation(%) | 5.3 | 5.1 | 5.6 | 5.9 | 2.7 | 4.4 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of mouse IL-12p70 into health mouse serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 104% to 118% with an overall mean recovery of 107%.
The recovery ranged from 104% to 118% with an overall mean recovery of 107%.
Sample Values
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
| Serum | 30 | 6.92-17.28 | 83 | 9.89 |
Serum/Plasma – Thirty samples from apparently healthy mice were evaluated for the presence of IL-12 p70 in this assay. No medical histories were available for the donors. n.d. = non-detectable. Samples measured below the sensitivity are considered to be non-detectable.
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