Mouse IL-17E ELISA Kit
Protocol Booklet
Key features and details
- Specification:
- Sensitivity:
- Standard Curve Range:
- Standard Curve Gradient:
- Number of Incubations:
- Sample Volume:
- Assay type:
- Operation Duration:
-
Brand:
CAT.NO. : AEM0028
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Size:
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Product Details
Background
The IL-17 family is comprised of at least six proinflammatory cytokines that share a conserved cysteine-knot structure but diverge at the N-terminus. IL-17 family members are glycoproteins secreted as dimers that induce local cytokine production and recruit granulocytes to sites of inflammation. IL-17 is induced by IL-15 and IL-23, mainly in activated CD4+ T cells distinct from Th1 or Th2 cells. IL-17F is the most homologous to IL-17, but is induced only by IL-23 in activated monocytes. IL-17B binds the IL-17B receptor, but not the IL-17 receptor; it is most homologous with IL-17D, which is expressed by resting CD4+ T cells and CD19+ B cells. IL-17E is mainly produced by Th2 cells and recruits eosinophils to lung tissue. IL-17C has a very restricted expression pattern but has been detected in adult prostate and fetal kidney libraries.
Typical data
| pg/ml | O.D. | Average | Corrected | |
| 0.00 | 0.0188 | 0.0195 | 0.0192 | |
| 41.15 | 0.0262 | 0.0264 | 0.0263 | 0.0072 |
| 123.46 | 0.0403 | 0.0407 | 0.0405 | 0.0214 |
| 370.37 | 0.0802 | 0.0801 | 0.0802 | 0.0610 |
| 1111.11 | 0.1814 | 0.1846 | 0.1830 | 0.1639 |
| 3333.33 | 0.4992 | 0.4831 | 0.4912 | 0.4720 |
| 10000.00 | 1.2740 | 1.2340 | 1.2540 | 1.2349 |
| 30000.00 | 2.8650 | 2.8640 | 2.8645 | 2.8454 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average(pg/ml) | 573.6 | 2641.9 | 7988.6 | 582.2 | 2756.5 | 8431 |
| Standard Deviation | 38.4 | 115.8 | 476.7 | 38 | 133.9 | 514,2 |
| Coefficient of Variation(%) | 6.7 | 4.4 | 6 | 6.5 | 4.9 | 6.1 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of mouse IL-17E into health mouse serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 103% to 122% with an overall mean recovery of 111%.
The recovery ranged from 103% to 122% with an overall mean recovery of 111%.
Sample Values
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
| Serum | 30 | n.d.-22.66 | 3 | 1.51 |
Serum/Plasma – Thirty samples from apparently healthy mice were evaluated for the presence of IL-17E in this assay. No medical histories were available for the donors. n.d. = non-detectable. Samples measured below the sensitivity are considered to be non-detectable.
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