Mouse IL-18 ELISA Kit
Protocol Booklet
Key features and details
- Specification:
- Sensitivity:
- Standard Curve Range:
- Standard Curve Gradient:
- Number of Incubations:
- Sample Volume:
- Assay type:
- Operation Duration:
-
Brand:
CAT.NO. : AEM0034
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Size:
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Product Details
Background
Interleukin 18 (IL-18), also known as interferon-gamma-inducing factor (IGIF) and IL-1 gamma, is a cytokine which shares biologic activities with IL-12 and structural similarities with the IL-1 family of proteins. IL-18 was originally cloned from liver cells and has since been shown to be expressed by monocyte/macrophages, osteoblasts and keratinocytes. Caspase-1 (IL-1 beta-converting enzyme) has been implicated in the physiological processing of pro-IL-18 to IL-18. Similarly to IL-12, human IL-18 has been shown to enhance NK cell activity in PBMC cultures. Human IL-18 has also been found to induce the production IFN-gamma and GM-CSF while inhibiting the production of IL-10 by PBMC. On enriched human T cells, human IL-18 can enhance Th1 cytokine production and stimulate cell proliferation via an IL-2-dependent pathway. In the mouse system, IL-18 has been shown to be a costimulatory factor for the activation of Th1, but not Th2, cells. IL-18 was found to selectively enhance the FasL-mediated cytotoxicity of Th1, but not Th0 or Th2, cells. IL-18 has also been shown to induce activated B cells to produce IFN-gamma that inhibits IgE production.
Typical data
| pg/ml | O.D. | Average | Corrected | |
| 0.00 | 0.0179 | 0.0183 | 0.0181 | |
| 13.72 | 0.0241 | 0.0221 | 0.0231 | 0.0050 |
| 41.15 | 0.0336 | 0.0318 | 0.0327 | 0.0146 |
| 123.46 | 0.0666 | 0.0613 | 0.0640 | 0.0459 |
| 370.37 | 0.1621 | 0.1576 | 0.1599 | 0.1418 |
| 1111.11 | 0.4508 | 0.4650 | 0.4579 | 0.4398 |
| 3333.33 | 1.4010 | 1.3650 | 1.3830 | 1.3649 |
| 10000.00 | 3.6290 | 3.5960 | 3.6125 | 3.5944 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average(pg/ml) | 190.3 | 892.4 | 3058.6 | 188.7 | 879.5 | 3032.9 |
| Standard Deviation | 6.1 | 33.9 | 144.5 | 5.9 | 30.7 | 139.6 |
| Coefficient of Variation(%) | 3.2 | 3.8 | 4.7 | 3.5 | 3.6 | 4.5 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of mouse IL-18 into health mouse serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 80% to 108% with an overall mean recovery of 94%.
The recovery ranged from 80% to 108% with an overall mean recovery of 94%.
Sample Values
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
| Serum | 30 | 85.35-284.06 | 100 | 163.41 |
Serum/Plasma – Thirty samples from apparently healthy mice were evaluated for the presence of CCL5 in this assay. No medical histories were available for the donors. n.d. = non-detectable. Samples measured below the sensitivity are considered to be non-detectable.
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