Mouse IL-21 ELISA Kit
Protocol Booklet
Key features and details
- Specification:
- Sensitivity:
- Standard Curve Range:
- Standard Curve Gradient:
- Number of Incubations:
- Sample Volume:
- Assay type:
- Operation Duration:
-
Brand:
CAT.NO. : AEM0020
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Size:
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Product Details
Background
IL-21 (Interleukin-21) is member of the family of cytokines that utilize the common gamma chain as a receptor subunit. This subunit is also a component of the receptors for IL-2, IL-4, IL-7, IL-9, and IL-15. IL-21 is produced by activated T follicular helper cells (Tfh), Th17 cells, and NKT cells. It exerts its biological effects through a heterodimeric receptor complex of the common gamma chain and the IL-21-specific IL-21 R. IL-21 supports Tfh cell development, immunoglobulin affinity maturation, plasma cell differentiation, B cell memory responses, and the migration of dendritic cells to draining lymph nodes. It costimulates the activation, proliferation, and survival of CD8+ T cells and NKT cells and promotes Th17 cell polarization. It blocks the generation of regulatory T cells and their suppressive effects on CD4+ T cells. In addition, IL-21 suppresses cutaneous hypersensitivity reactions by limiting allergen-specific IgE production and mast cell degranulation.
Typical data
| pg/ml | O.D. | Average | Corrected | |
| 0.00 | 0.0521 | 0.0511 | 0.0516 | |
| 27.43 | 0.0672 | 0.0643 | 0.0658 | 0.0142 |
| 82.30 | 0.0875 | 0.0876 | 0.0876 | 0.0360 |
| 246.91 | 0.1633 | 0.1654 | 0.1644 | 0.1128 |
| 740.74 | 0.3352 | 0.3411 | 0.3382 | 0.2866 |
| 2222.22 | 0.7811 | 0.8421 | 0.8116 | 0.7600 |
| 6666.67 | 1.8321 | 1.9812 | 1.9067 | 1.8551 |
| 20000.00 | 3.3582 | 3.6173 | 3.4878 | 3.4362 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average(pg/ml) | 283.7 | 1762.5 | 5986 | 298.1 | 1790.8 | 6344.1 |
| Standard Deviation | 15.7 | 81.7 | 333.9 | 18.1 | 88.3 | 358.4 |
| Coefficient of Variation(%) | 5.5 | 4.6 | 5.6 | 6.1 | 4.9 | 5.6 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of mouse IL-21 into health mouse serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 84% to 106% with an overall mean recovery of 92%.
The recovery ranged from 84% to 106% with an overall mean recovery of 92%.
Sample Values
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
| Serum | 30 | n.d.-41.85 | 39 | 26.91 |
Serum/Plasma – Thirty samples from apparently healthy mice were evaluated for the presence of IL-21 in this assay. No medical histories were available for the donors. n.d. = non-detectable. Samples measured below the sensitivity are considered to be non-detectable.
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