Mouse IL12 p70 ELISA Kit
Key features and details
- 产品描述:
- 反应物种:
- 应用:
- 特异性:
- 靶点名称:
- 偶联标记:
- 偶联标记说明:
- 灵敏度:
- 样品类型:
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Brand:
CAT.NO. : ARG80201
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Product Details
内含物
| 货号 | 内含物名称 | 包装 | 温度 |
|---|---|---|---|
| ARG80201-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG80201-002 | Standard (Lyophilized) | 3 X 1 ng/vial | 4°C |
| ARG80201-003 | Standard diluent buffer | 20 ml | 4°C |
| ARG80201-004 | Antibody conjugate concentrate | 400 µl | 4°C |
| ARG80201-005 | Antibody diluent buffer | 16 ml | 4°C |
| ARG80201-006 | HRP-Streptavidin concentrate | 400 µl | 4°C (Protect from light) |
| ARG80201-007 | HRP-Streptavidin diluent buffer | 16 ml | 4°C |
| ARG80201-008 | 20X Wash buffer | 50 ml | 4°C |
| ARG80201-009 | TMB substrate | 12ml | 4°C (Protect from light) |
| ARG80201-010 | STOP solution | 12ml | 4°C |
| ARG80201-011 | Plate sealer | 4 strips | Room temperature |
概述
| 产品描述 | ARG80201 Mouse IL12 p70 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Mouse IL12 p70 in Serum, Plasma, Cell culture supernatants. |
|---|---|
| 反应物种 | Ms |
| 应用 | ELISA |
| 特异性 | No significant cross-reactivity or interference with Mouse IL-12 p35, IL-12/IL-23 p40 monomer, IL-12/IL-23 p40 dimer, IL-23；human IL-12 p70, IL-12 p35, IL-12/IL-23 p40 monomer；canine IL-12 p70；feline IL-12 p70 |
| 靶点名称 | IL12 p70 |
| 偶联标记 | HRP |
| 偶联标记说明 | Substrate: TMB and read at 450 nm |
| 灵敏度 | 8 pg/ml |
| 样品类型 | Serum, Plasma, Cell culture supernatants |
| 标准范围 | 15.6 - 1000 pg/ml |
| 样本量 | 100 µl |
| 精确 | CV: less than10% |
| 別名 | IL12A; Interleukin 12A; IL-12A; NKSF1; CLMF; NFSK; P35; Interleukin 12A (Natural Killer Cell Stimulatory Factor 1, Cytotoxic Lymphocyte Maturation Factor 1, P35); Cytotoxic Lymphocyte Maturation Factor 35 KDa Subunit; Cytotoxic Lymphocyte Maturation Factor 1, P35; NF Cell Stimulatory Factor Chain 1; NK Cell Stimulatory Factor Chain 1; Interleukin-12 Subunit Alpha; Interleukin-12 Alpha Chain; Interleukin 12, P35; IL-12, Subunit P35; IL35 Subunit; CLMF P35; Natural Killer Cell Stimulatory Factor 1, 35 KD Subunit; IL-12 Subunit P35 |
应用说明
| 检测时间 | 4 hours |
|---|
属性
| 形式 | 96 well |
|---|---|
| 存放说明 | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | Il12a |
| 全名 | interleukin 12a |
| 背景介绍 | Interleukin-12 is a disulfide-linked heterodimeric protein (p70), composed of two subunits, p35 and p40, which are encoded by two different genes. Accumulating data indicate that p40 secretion precedes that of IL-12 expression. In addition, to its ability to covalently bind to p35 to form IL-12, p40 can bind to p19 to form IL-23. Additionally, p40 can associate itself through disulfide linkage to form a dimer that acts as a potent IL12 antagonist. Recombinant murine IL-12 p40 is a 40.0 kDa protein containing 313 amino acid residues. |
| 产品亮点 | Related products: IL12 p70 ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| 研究领域 | Immune System kit |
检测图片 (1)
ARG80201 Mouse IL12 p70 ELISA Kit standard curve image
ARG80201 Mouse IL12 p70 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
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