Mouse JAM-C ELISA Kit
Key features and details
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- 偶联标记:
- 偶联标记说明:
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Brand:
CAT.NO. : ARG82400
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Product Details
内含物
| 货号 | 内含物名称 | 包装 | 温度 |
|---|---|---|---|
| ARG82400-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG82400-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG82400-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG82400-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82400-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82400-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82400-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82400-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG82400-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG82400-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG82400-011 | Plate sealer | 4 strips | Room temperature |
概述
| 产品描述 | ARG82400 Mouse JAM-C ELISA Kit is an Enzyme Immunoassay kit for the quantification of Mouse JAM-C in serum, plasma (EDTA, heparin) and cell culture supernatants. |
|---|---|
| 反应物种 | Ms |
| 应用 | ELISA |
| 靶点名称 | JAM-C |
| 偶联标记 | HRP |
| 偶联标记说明 | Substrate: TMB and read at 450 nm. |
| 灵敏度 | 15.6 pg/ml |
| 样品类型 | Serum, plasma (EDTA, heparin) and cell culture supernatants. |
| 标准范围 | 31.2 - 2000 pg/ml |
| 样本量 | 100 µl |
| 精确 | Intra-Assay CV: 4.9% Inter-Assay CV: 6.2% |
| 別名 | JAM-2; JAM-3; Junctional adhesion molecule C; JAM-C; Junctional adhesion molecule 3; JAMC |
应用说明
| 检测时间 | ~ 5 hours |
|---|
属性
| 形式 | 96 well |
|---|---|
| 存放说明 | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | JAM3 |
| 全名 | junctional adhesion molecule 3 |
| 背景介绍 | Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. The protein encoded by this immunoglobulin superfamily gene member is localized in the tight junctions between high endothelial cells. Unlike other proteins in this family, the this protein is unable to adhere to leukocyte cell lines and only forms weak homotypic interactions. The encoded protein is a member of the junctional adhesion molecule protein family and acts as a receptor for another member of this family. A mutation in an intron of this gene is associated with hemorrhagic destruction of the brain, subependymal calcification, and congenital cataracts. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Apr 2011] |
| 生物功能 | Participates in cell-cell adhesion. It is a counter-receptor for ITGAM, mediating leukocyte-platelet interactions and is involved in the regulation of transepithelial migration of polymorphonuclear neutrophils (PMN). The soluble form is a mediator of angiogenesis. [UniProt] |
| 细胞定位 | Cell membrane; Single-pass type I membrane protein. Cell junction, desmosome. Secreted, extracellular space. Note=In epithelial cells, it is expressed at desmosomes but not at tight junctions (PubMed:15194813). Localizes at the cell surface of endothelial cells; treatment of endothelial cells with vascular endothelial growth factor stimulates recruitment of JAM3 to cell-cell contacts (PubMed:15994945). [UniProt] |
| 产品亮点 | Related products: JAM-C antibodies; JAM-C ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| 翻译后修饰 | Proteolytically cleaved from endothelial cells surface into a soluble form by ADAM10 and ADAM17; the release of soluble JAM3 is increased by proinflammatory factors. [UniProt] |
检测图片 (1)
ARG82400 Mouse JAM-C ELISA Kit standard curve image
ARG82400 Mouse JAM-C ELISA Kit results of a typical standard run with optical density reading at 450 nm.
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