Mouse MFG-E8 / Lactadherin ELISA Kit
Key features and details
- 产品描述:
- 反应物种:
- 应用:
- 特异性:
- 靶点名称:
- 偶联标记:
- 偶联标记说明:
- 灵敏度:
- 样品类型:
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Brand:
CAT.NO. : ARG81845
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Product Details
内含物
| 货号 | 内含物名称 | 包装 | 温度 |
|---|---|---|---|
| ARG81845-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81845-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG81845-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81845-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81845-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81845-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81845-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81845-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81845-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81845-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81845-011 | Plate sealer | 4 strips | Room temperature |
概述
| 产品描述 | ARG81845 Mouse MFG-E8 / Lactadherin ELISA Kit is an Enzyme Immunoassay kit for the quantification of Mouse MFG-E8 / Lactadherin in serum, plasma (heparin, EDTA) and cell culture supernatants. |
|---|---|
| 反应物种 | Ms |
| 应用 | ELISA |
| 特异性 | There is no detectable cross-reactivity with other relevant proteins. |
| 靶点名称 | MFGE8 / Lactadherin |
| 偶联标记 | HRP |
| 偶联标记说明 | Substrate: TMB and read at 450 nm. |
| 灵敏度 | 31.25 pg/ml |
| 样品类型 | Serum, plasma (heparin, EDTA) and cell culture supernatants. |
| 标准范围 | 62.5 - 4000 pg/ml |
| 样本量 | 100 µl |
| 精确 | Intra-Assay CV: 6.4%; Inter-Assay CV: 7.4% |
| 別名 | SPAG10; MFGM; HsT19888; HMFG; Milk fat globule-EGF factor 8; MFG-E8; BA46; hP47; EDIL1; Lactadherin; OAcGD3S; Breast epithelial antigen BA46; SED1 |
应用说明
| 检测时间 | ~ 5 hours |
|---|
属性
| 形式 | 96 well |
|---|---|
| 存放说明 | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | MFGE8 |
| 全名 | milk fat globule-EGF factor 8 protein |
| 背景介绍 | This gene encodes a preproprotein that is proteolytically processed to form multiple protein products. The major encoded protein product, lactadherin, is a membrane glycoprotein that promotes phagocytosis of apoptotic cells. This protein has also been implicated in wound healing, autoimmune disease, and cancer. Lactadherin can be further processed to form a smaller cleavage product, medin, which comprises the major protein component of aortic medial amyloid (AMA). Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2015] |
| 生物功能 | Plays an important role in the maintenance of intestinal epithelial homeostasis and the promotion of mucosal healing. Promotes VEGF-dependent neovascularization (By similarity). Contributes to phagocytic removal of apoptotic cells in many tissues. Specific ligand for the alpha-v/beta-3 and alpha-v/beta-5 receptors. Also binds to phosphatidylserine-enriched cell surfaces in a receptor-independent manner. Zona pellucida-binding protein which may play a role in gamete interaction. Binds specifically to rotavirus and inhibits its replication. Medin is the main constituent of aortic medial amyloid. [UniProt] |
| 产品亮点 | Related products: MFGE8 antibodies; MFGE8 ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| 翻译后修饰 | Medin has a ragged N-terminus with minor species starting at Pro-264 and Gly-273. [UniProt] |
检测图片 (1)
ARG81845 Mouse MFG-E8 / Lactadherin ELISA Kit standard curve image
ARG81845 Mouse MFG-E8 / Lactadherin ELISA Kit results of a typical standard run with optical density reading at 450 nm.
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