Mouse NBL1 ELISA Kit
Key features and details
- 产品描述:
- 反应物种:
- 应用:
- 特异性:
- 靶点名称:
- 偶联标记:
- 偶联标记说明:
- 灵敏度:
- 样品类型:
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Brand:
CAT.NO. : ARG81729
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Product Details
内含物
| 货号 | 内含物名称 | 包装 | 温度 |
|---|---|---|---|
| ARG81729-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81729-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG81729-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81729-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81729-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81729-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81729-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81729-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81729-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81729-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81729-011 | Plate sealer | 4 strips | Room temperature |
概述
| 产品描述 | ARG81729 Mouse NBL1 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Mouse NBL1 in serum, plasma (heparin, EDTA) and cell culture supernatants. |
|---|---|
| 反应物种 | Ms |
| 应用 | ELISA |
| 特异性 | There is no detectable cross-reactivity with other relevant proteins. |
| 靶点名称 | NBL1 |
| 偶联标记 | HRP |
| 偶联标记说明 | Substrate: TMB and read at 450 nm. |
| 灵敏度 | 31.25 pg/ml |
| 样品类型 | Serum, plasma (heparin, EDTA) and cell culture supernatants. |
| 标准范围 | 62.5 - 4000 pg/ml |
| 样本量 | 100 µl |
| 精确 | Intra-Assay CV: 5.7%; Inter-Assay CV: 6.7% |
| 別名 | NB; DAN; NO3; DAND1; D1S1733E |
应用说明
| 检测时间 | ~ 5 hours |
|---|
属性
| 形式 | 96 well |
|---|---|
| 存放说明 | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | Swiss-port # Q61477 Mouse Neuroblastoma suppressor of tumorigenicity 1 |
|---|---|
| 基因名称 | NBL1 |
| 全名 | NBL1, DAN family BMP antagonist |
| 背景介绍 | This gene product is the founding member of the evolutionarily conserved CAN (Cerberus and DAN) family of proteins, which contain a domain resembling the CTCK (C-terminal cystine knot-like) motif found in a number of signaling molecules. These proteins are secreted, and act as BMP (bone morphogenetic protein) antagonists by binding to BMPs and preventing them from interacting with their receptors. They may thus play an important role during growth and development. Alternatively spliced transcript variants have been identified for this gene. Read-through transcripts between this locus and the upstream mitochondrial inner membrane organizing system 1 gene (GeneID 440574) have been observed. [provided by RefSeq, May 2013] |
| 生物功能 | Possible candidate as a tumor suppressor gene of neuroblastoma. May play an important role in preventing cells from entering the final stage (G1/S) of the transformation process. [UniProt] |
| 产品亮点 | Related products: NBL1 antibodies; NBL1 ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
检测图片 (1)
ARG81729 Mouse NBL1 ELISA Kit standard curve image
ARG81729 Mouse NBL1 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
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