Mouse PD-L1 ELISA Kit

Key features and details

Specification: 96 Test
Sensitivity: 0.16 pg/ml (10 μl)
Standard Curve Range: 1.37~1000 pg/ml
Standard Curve Gradient: 7 Points/3 Folds
Number of Incubations: 2
Sample Volume: 10 μl
Assay type: Sandwich Elisa
Operation Duration: 120min

Brand:

CAT.NO. : AEM0036

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Size:

96T

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Product Details

Background

PD-L1, also known as B7-H1 and CD274, is an approximately 65 kDa transmembrane glycoprotein in the B7 family of immune regulatory molecules. PD-L1 is expressed on inflammatory-activated immune cells including macrophages, T cells, and B cells, keratinocytes, enothelial and intestinal epithelial cells, as well as a variety of carcinomas and melanoma. PD-L1 binds to T cell B7-1/CD80 and PD-1. It suppresses T cell activation and proliferation and induces the apoptosis of activated T cells. It plays a role in the development of immune tolerance by promoting T cell anergy and enhancing regulatory T cell development. PD-L1 favors the development of anti-inflammatory IL-10 and IL-22 producing dendritic cells and inhibits the development of Th17 cells. In cancer, PD-L1 provides resistance to T cell mediated lysis, enhances EMT, and enhances the tumorigenic function of Th22 cells.

Typical Data

pg/ml	O.D.		Average	Corrected
0.00	0.0578	0.0568	0.0573
1.37	0.0794	0.0801	0.0798	0.0225
4.12	0.1089	0.0990	0.1040	0.0467
12.35	0.1731	0.1708	0.1720	0.1147
37.04	0.3835	0.3865	0.3850	0.3277
111.11	0.9157	0.9152	0.9155	0.8582
333.33	2.1664	2.1622	2.1643	2.1070
1000.00	3.9609	3.9586	3.9598	3.9025

Precision

	Intra-assay Precision			Inter-assay Precision
Sample Number	S1	S2	S3	S1	S2	S3
Sample Number	22	22	22	6	6	6
Average(pg/ml)	24.1	109.5	371.6	24.4	127.7	359.1
Standard Deviation	0.8	6.8	8.5	1.0	3.9	6.9
Coefficient of Variation(%)	3.3	6.2	2.3	4.0	3.1	1.9

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.

Spike Recovery

The spike recovery was evaluated by spiking 3 levels of mouse PD-L1 into health mouse serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 86% to 116% with an overall mean recovery of 107%.

Sample Values

Sample Matrix	Sample Evaluated	Range (pg/ml)	Detectable (%)	Mean of Detectable (pg/ml)
Serum	30	122.17-529.36	100	341.58

Serum/Plasma – Thirty samples from apparently healthy mice were evaluated in this assay. Donor medical histories were not available.

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