Mouse Phospholipase A2 IIA ELISA Kit
Key features and details
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- 偶联标记:
- 偶联标记说明:
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Brand:
CAT.NO. : ARG82429
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Product Details
内含物
| 货号 | 内含物名称 | 包装 | 温度 |
|---|---|---|---|
| ARG82429-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG82429-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG82429-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG82429-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82429-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82429-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82429-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82429-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG82429-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG82429-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG82429-011 | Plate sealer | 4 strips | Room temperature |
概述
| 产品描述 | ARG82429 Mouse Phospholipase A2 IIA ELISA Kit is an Enzyme Immunoassay kit for the quantification of Mouse Phospholipase A2 IIA in serum, plasma (EDTA, heparin) and cell culture supernatants. |
|---|---|
| 反应物种 | Ms |
| 应用 | ELISA |
| 靶点名称 | Phospholipase A2 IIA |
| 偶联标记 | HRP |
| 偶联标记说明 | Substrate: TMB and read at 450 nm. |
| 灵敏度 | 15.6 pg/ml |
| 样品类型 | Serum, plasma (EDTA, heparin) and cell culture supernatants. |
| 标准范围 | 31.2 - 2000 pg/ml |
| 样本量 | 100 µl |
| 精确 | Intra-Assay CV: 5.0% Inter-Assay CV: 5.8% |
| 別名 | PLA2B; GIIC sPLA2; EC 3.1.1.4; Non-pancreatic secretory phospholipase A2; PLA2; MOM1; PLA2L; PLAS1; PLA2S; NPS-PLA2; Group IIA phospholipase A2; sPLA2; Phospholipase A2, membrane associated; Phosphatidylcholine 2-acylhydrolase 2A |
应用说明
| 检测时间 | ~ 5 hours |
|---|
属性
| 形式 | 96 well |
|---|---|
| 存放说明 | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | Swiss-port # P31482 Mouse Phospholipase A2, membrane associated |
|---|---|
| 基因名称 | PLA2G2A |
| 全名 | phospholipase A2, group IIA (platelets, synovial fluid) |
| 背景介绍 | The protein encoded by this gene is a member of the phospholipase A2 family (PLA2). PLA2s constitute a diverse family of enzymes with respect to sequence, function, localization, and divalent cation requirements. This gene product belongs to group II, which contains secreted form of PLA2, an extracellular enzyme that has a low molecular mass and requires calcium ions for catalysis. It catalyzes the hydrolysis of the sn-2 fatty acid acyl ester bond of phosphoglycerides, releasing free fatty acids and lysophospholipids, and thought to participate in the regulation of the phospholipid metabolism in biomembranes. Several alternatively spliced transcript variants with different 5' UTRs have been found for this gene.[provided by RefSeq, Sep 2009] |
| 生物功能 | Thought to participate in the regulation of the phospholipid metabolism in biomembranes including eicosanoid biosynthesis. Catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides. [UniProt] |
| 细胞定位 | Cell membrane; Peripheral membrane protein. Secreted. [UniProt] |
| 产品亮点 | Related products: Phospholipase antibodies; Phospholipase ELISA Kits; Phospholipase Duos / Panels; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
检测图片 (1)
ARG82429 Mouse Phospholipase A2 IIA ELISA Kit standard curve image
ARG82429 Mouse Phospholipase A2 IIA ELISA Kit results of a typical standard run with optical density reading at 450 nm.
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