Mouse Pro-collagen I alpha 1/COL1A1 ELISA Kit
Key features and details
- Specification:
- Sensitivity:
- Standard Curve Range:
- Standard Curve Gradient:
- Number of Incubations:
- Sample Volume:
- Assay type:
- Operation Duration:
-
Brand:
CAT.NO. : AEMY0045
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Product Details
Background
Type I collagen is the most abundant structural protein of connective tissues such as skin, bone and tendon. It is synthesized as a procollagen molecule which is characterized by a 300 nm triple helical domain flanked by globular N- and C-terminal propeptides. The triple helical domain contains Gly-Xaa-Yaa triplets where Xaa and Yaa are frequently proline and hydroxyproline, respectively. The non-helical propeptides are removed by procollagen N- and C-proteinase activities so that the mature triple helices can self-assemble into collagen fibrils that provide tensile strength to tissues. Type I collagen is a heterotrimer that consists of two alpha 1(I) chains and one alpha 2(I) chain, although homotrimers consisting of three identical alpha 1(I) chains have also been described. This recombinant mini pro-alpha 1(I) collagen consists of a shortened alpha 1(I) chain with following domain structure from N- to C-terminus: N-propeptide, N‑telopeptide, the 33 most N-terminal Gly-Xaa-Yaa repeats, the 33 most C-terminal Gly-Xaa-Yaa repeats, C-telopeptide and C-propeptide. The preparation contains a mixture of the full-length molecule, pN collagen I( alpha 1) and the C-terminal propeptide. This truncated pro-alpha 1(I) collagen is a substrate for procollagen N-proteinase and procollagen C-proteinase.
Typical data
|
ng/ml |
O.D. |
Average |
Corrected |
|
|
0.00 |
0.0059 |
0.0063 |
0.0061 |
|
|
0.27 |
0.0196 |
0.0204 |
0.0200 |
0.0139 |
|
0.82 |
0.0474 |
0.0530 |
0.0502 |
0.0441 |
|
2.47 |
0.1412 |
0.1520 |
0.1466 |
0.1405 |
|
7.41 |
0.4025 |
0.4208 |
0.4117 |
0.4056 |
|
22.22 |
1.1140 |
1.2170 |
1.1655 |
1.1594 |
|
66.67 |
2.8660 |
2.8730 |
2.8695 |
2.8634 |
|
200.00 |
4.1954 |
4.3502 |
4.2728 |
4.2667 |
Precision
|
Intra-assay Precision |
Inter-assay Precision |
|||||
|
Sample Number |
S1 |
S2 |
S3 |
S1 |
S2 |
S3 |
|
22 |
22 |
22 |
6 |
6 |
6 |
|
|
Average(ng/ml) |
5.4 |
26.4 |
74.1 |
4.0 |
21.4 |
79.3 |
|
Standard Deviation |
0.4 |
2.1 |
4.3 |
0.2 |
0.4 |
4.1 |
|
Coefficient of Variation(%) |
6.7 |
7.8 |
5.8 |
4.8 |
2.0 |
5.1 |
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of Mouse Pro-collagen I alpha 1/COL1A1 into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 86% to 119% with an overall mean recovery of 103%.
The recovery ranged from 86% to 119% with an overall mean recovery of 103%.
Sample Values
| Sample Matrix | Sample Evaluated | Range (ng/ml) | Detectable (%) | Mean of Detectable (ng/ml) |
|---|---|---|---|---|
| Serum | 30 | 146.01-320.89 | 100 | 223.31 |
Serum/Plasma – Thirty samples from apparently healthy mice were evaluated for the presence of Pro-collagen I alpha 1/COL1A1 in this assay. No medical histories were available for the donors.
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