Mouse/Rat IGF1/IGF-I/IGF-1 ELISA Kit

Protocol Booklet

Key features and details

Specification: 96 Test
Sensitivity: 1.76 pg/ml (50 μl)；3.43 pg/ml (10 μl)
Standard Curve Range: 6.86~5000 pg/ml
Standard Curve Gradient: 7 Points/3 Folds
Number of Incubations: 2
Sample Volume: 50 μl/10 μl
Assay type: Sandwich Elisa
Operation Duration: 120min

Brand:

CAT.NO. : AEM0024

RMB Please choose

Size:

96T

Trail, Bulk size or Custom requests Please contact us

*产品价格可能会有所调整，请以品牌方官网实时更新的价格为准，以确保准确性。

Product Details

Background

IGF-I receptor is a disulfide-linked heterotetrameric transmembrane protein consisting of two alpha and two beta subunits. Both the alpha and beta subunits are encoded within a single receptor precursor cDNA. The proreceptor polypeptide is proteolytically cleaved and disulfide-linked to yield the mature heterotetrameric receptor. The alpha subunit of IGF-I receptor is extracellular while the beta subunit has an extracellular domain, a transmembrane domain and a cytoplasmic tyrosine kinase domain. The IGF-I receptor is highly expressed in all cell types and tissues. - IGF-II R is a type I transmembrane glycoprotein that contains a 2,264 amino acid (aa) extracellular region, a 23 aa transmembrane segment segment and a 124 aa cytoplasmic tail. IGF-II R regulates many diverse biological functions that range from intracellular trafficking to the internalization of extracellular factors and modulation of cellular responses. It delivers newly synthesized M6P-tagged lysosomal enzymes from the trans-golgi network to endosomes, and facilitates the clearance of extracellular lysosomal and matrix degrading enzymes by internalization into clathrin-coated vesicles and delivery into endosomes. With respect to IGF-II biology, It would appear that IGF-II R is principally a regulator of local IGF-II levels, targeting IGF-II for destruction in lysosomes. - The heterotetrameric receptors for insulin (INS R) and IGF-I (IGF-I R) are receptor tyrosine kinases that consist of two ligandbinding alpha subunits and two beta subunits. Ligand binding induces autophosphorylation on multiple tyrosine residues of beta subunits. Phosphorylation of Tyr1162 and 1163 on INS R and Tyr1135 and 1136 on IGF-I R stimulates intrinsic kinase activity.

Typical data

pg/ml	O.D.		Average	Corrected
0.00	0.0647	0.0635	0.0641
6.86	0.1385	0.1303	0.1344	0.0703
20.58	0.2735	0.2724	0.2730	0.2089
61.73	0.5618	0.5279	0.5449	0.4808
185.19	1.0550	0.9549	1.0050	0.9409
555.56	1.6270	1.5390	1.5830	1.5189
1666.67	2.2390	2.0130	2.1260	2.0619
5000.00	2.6320	2.6820	2.6570	2.5930

Precision

	Intra-assay Precision			Inter-assay Precision
Sample Number	S1	S2	S3	S1	S2	S3
Sample Number	22	22	22	6	6	6
Average(pg/ml)	119.3	509	1409.9	109.9	510.1	1498.1
Standard Deviation	8.2	39.3	109.4	5.4	27	100.4
Coefficient of Variation(%)	6.9	7.7	7.8	4.9	5.3	6.7

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.

Spike Recovery

The spike recovery was evaluated by spiking 3 levels of Mouse/Rat IGF-I/IGF-1 into health mouse serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 94% to 105% with an overall mean recovery of 101%.

Sample Values

Sample Matrix

Sample

Evaluated

Range

(ng/ml)

Detectable

Mean of Detectable

(ng/ml)

Serum

4.93-460.32

100

135.06

Serum/Plasma - Thirty samples from apparently healthy mice were evaluated for the presence of Mouse/Rat IGF-I in this assay. No medical histories were available for the donors.

Sample Matrix

Sample

Evaluated

Range

(ng/ml)

Detectable

Mean of Detectable

(ng/ml)

Serum

84.54-1888.06

100

584.19

Serum/Plasma - Thirty samples from apparently healthy rats were evaluated for the presence of Mouse/Rat IGF-I in this assay. No medical histories were available for the donors.

New Products

IDH1 Rabbit Monoclonal Antibody(ARA946)

S100 Rabbit Monoclonal Antibody(ARA945)

p63 Rabbit Monoclonal Antibody(ARA944)

Cytokeratin 5&6 Rabbit Monoclonal Antibody(ARA943)

TTF1 Rabbit Monoclonal Antibody(ARA942)

BCL-2 Rabbit Monoclonal Antibody(ARA941)